Page 7 - Shimadzu Journal vol.5 Issue1

Glycan Analysis

Table 1 The m/z of four oligosaccharides, G1-G4, and their oxidized products.
The MS spectra showed the reaction product under different oxidation conditions.

Monitoring the Oxidation Reaction Conditions Using MS Detection
n
+
(M+H ) G1 G2 G3 G4

Four oligosaccharides 933.318 1339.476 1663.582 2273.773
One monosaccharide oxidized 931.302 N/A 1661.566 2241.731
Two monosaccharides oxidized 929.286 N/A 1659.550 2239.715
Three monosaccharides oxidized N/A N/A N/A 2211.705
Four monosaccharides oxidized N/A N/A N/A 2209.689

Reaction conditions: 100 933.24 100 1339.37 100 1663.54 100 2273.51
80 80 80 80
60 60 60 60
40 40 40 40
Four oligosaccharides
before oxidation 20 20 20 20
100 100 100 100
933.26 1339.40 1663.49 2273.74
80 80 80 80
2225.66
Mild condition: 60 60 60 60 2255.67
1 mM NaIO 4 , 40 40 40 40 2241.69
on the ice, 20 931.23 20 20 20 2211.64
15 min in the dark
100 100 100 100
931.28 1339.44 1661.54 1663.55 2225.73 2255.71
80 80 1337.43 80 80
929.26
60 60 60 60 2241.76 2273.73
933.29
2211.72
Oxidation with 40 40 40 1659.53 40
10 mM NaIO 4 , 4ºC, 20 20 20 20
60 min in the dark 0 0 0 0
925 927 933 935 937 939 941 943 1335 1337 1339 1341 1343 1345 1347 1655 1657 1659 1661 1663 1665 1667 1669 2200 2220 2240 2260 2280 2300
m/z m/z m/z m/z
Fig. 3 The MS spectra has shown the reaction products under different oxidation conditions

Enlargement of
fragment peaks

MS2_1337

MS2_1339

Notes: The fragment ion
peak 507 indicated one MS3_1339_1136
monosaccharide has been
oxidized at man-man-man
structure. However, It
can’t identified which
mannose has been
oxidized based on current
stage.

Fig. 4 Identify glycan structures of 1337 and 1339 using MS n

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