Page 17 - Shimadzu Journal vol.3 Issue3

Drug Discovery

3. Results
(x100,000)
478.322
447.282 461.296
450.292
2.5
433.267
506.137
430.266 480.154
500.301
0.0
425.0 450.0 475.0 500.0
m/z
MS spectrum of standard gentamicin C 1 (2 mg/mL, CHCA)
(x100,000)
322.196
2.0

1.0

461.296
0.0
50 100 150 200 250 300 350 400 450 500
m/z
MS/MS spectrum of standard gentamicin C 1 (m/z 478.322)

Data acquisition was performed in product ion scan (MS/MS) mode
and abundance of gentamicin C 1 was selectively monitored by
focusing on its specific fragment ion, m/z 322.196.

Optimization of matrix application method
1) Spray 2) Sublimation + Spray
Analyte
Matrix
Tissue section
microscope
glass plate
3) Sublimation + Nebulizer *) 4) Pre-coated matrix + Sublimation + Nebulizer

Matrix (sublimation)
Matrix (pre-coated)

*: S. Shimma et al, J. Mass Spectrom., 48: 1285–1290, 2013.

1) Peak intensity: 12,731 2) Peak intensity: 24,291

3) Peak intensity: 154,514 4) Peak intensity: 239,536

The peak intensities of gentamicin C1 by Method 4) rose 20 times from those by Method 1).
m/z: 478.3 ˠ 322.2

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