Page 20 - Shimadzu Journal vol.3 Issue3

Posters from These articles were selected by Shimadzu. Relating to pharmaceutical analysis, they are from posters
Recent presented at recent international conferences in 2015, such as ASMS and HPLC. They feature a variety of
instruments we produce and include cutting-edge technologies. Please obtain the articles of your
Conferences interest through the links on the titles.

Selection 1 Pharmaceutical, Life Science
A Fully Automated, Bottom-up Approach for MALDI-TOF MS Based Discovery Workflows
Bottom-up workflows have been a staple of mass spectrometry-based proteomic approaches. Most of these protocols require overnight
digestion, sample clean-up and, when involving MALDI, fraction collection, dry down and sample matrix deposition. We present in this
work a fully automated solution for MALDI-TOF MS-based peptide mapping experiments. Using an online digestion, desalt, reversed
phase separation and fraction collection platform, we were able to decrease the experimental time from over 18 hours to less than 30
min (reversed phase separation included, acquisition time not included) while substantially improving sequence coverage.

Selection 2 Pharmaceutical, Life Science
Application of a Sensitive Liquid Chromatography-Tandem Mass Spectrometric Method for
Determination of Octreotide in Human Plasma
A fast and sensitive method for detecting octreotide in human plasma by ultra high performance liquid chromatography-tandem
mass spectrometry was developed. The lower limit of quantitation was 5.0 pg/mL and the results of other parameters for method
validation were good.

Selection 3 Pharmaceutical, Life Science
Characterization of Products Formed by Forced Degradation of Etodolac Using LC/MS/MS
Forced degradation is a process, whereby the natural degradation rate of a drug product or drug substance is accelerated by the
application of an additional stress. The ICH guidelines indicate that stress testing is designed to determine the stability of the
molecule by knowing degradation pathways in order to identify the likely degradation products. In this work, acid, base, peroxide,
thermal and UV degradation products of Etodolac were studied using the LCMS-8040 Triple Quadrupole Mass Spectrometer.

Selection 4 Pharmaceutical, Life Science
Comprehensive Two-Dimensional HPLC Analysis Coupled with Mass Spectrometric Detection and
Informative Data Processing for Lipid Analysis
In lipidomics, phospholipids are the attractive targets of analysis since lipids are important and essential components of biological
membranes. However, a conventional HPLC system with a single separation mode performs poorly on biological lipid sample,
because it contains various kinds of lipids with common moieties that govern their behavior on column. In such a case,
comprehensive two-dimensional (2D) LC will be a powerful tool.

Selection 5 Pharmaceutical, Life Science
Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids
Kakkonto, a traditional Chinese drug, consists of natural plants and contains many compounds such as ephedrine, glycyrrhizic
acid, and cinnamic acid. We separated such a complex matrix using the Shimadzu Nexera-e comprehensive two-dimensional liquid
chromatograph, with a photodiode array detector. We also characterized phospholipids using Nexera-e coupled with triple
quadrupole and ion-trap-TOF mass spectrometers quantitatively and qualitatively, respectively.

Selection 6 Pharmaceutical, Life Science
Determination of Leachables in Orally Inhaled and Nasal Drug Products (OINDP) by GCMS/MS
Orally Inhaled and Nasal Drug Products (OINDP) are developed for treating asthma or chronic obstructive pulmonary diseases by
delivering drug substances directly to the respiratory tract. OINDPs are present in inhaler devices, which may contain polymers,
elastomers and other components from which minute quantities of material may migrate/leach into a product and enter the
respiratory tract along with the therapeutic agent. In this study, we tried to develop a highly sensitive method for quantitation of
40 leachables in OINDP products by using the Shimadzu GCMS-TQ8040.
Selection 7 Pharmaceutical, Life Science
Development of Simplified Quantitative Method for Tryptic Digested C-reactive Protein by
Using Online SPE Coupled to Triple Quadrupole Mass Spectrometer
Method development for accurate quantification of biomarker protein is a very important step for researchers in pharmaceutical
and clinical fields. In this study, we tried to develop a simple and high-sensitivity quantification method for C reactive protein
(CRP), the level of which rises as a result of in ammation etc., by using online SPE coupled to a triple quadrupole mass
spectrometer without any complicated sample enrichment and depletion.

Selection 8 Pharmaceutical, Life Science
Direct Analysis of 10 Antipsychotics in Serum by the Online System Integrating Solid-Phase Extraction with UHPLC-MS/MS
A fully-automatic system integrating solid-phase extraction with ultra-high performance liquid chromatography-tandem mass
spectrometry was successfully applied to the direct analysis of ten antipsychotics in serum.