Page 19 - Shimadzu Journal vol.3 Issue3

Drug Discovery

Precise Localization of Gentamicin in Renal Cortex
The image of the specific distribution of gentamicin in proximal renal tubules of rats was detected with a high resolution of 10 µm (pixel size),
and found to be similar to the distribution of the immunostaining assay of gentamicin reported previously*.
*: K. Fujiwara, Yakugaku Zasshi, 131(6): 949-960, 2011
Pixel size: 10 µm
Optical image (H&E stained) Overlap image MALDI image
100 µm

Strong

Weak

Blue boundary: glomerulus, Yellow boundary: distal renal tubules
The signal is not detected in these domains.

4. Conclusions 5. Acknowledgments
High resolution (10 µm) imaging of rat renal cortex showed that This work was supported by Safety Research Laboratories of
gentamicin is specifically distributed in proximal renal tubules. Mitsubishi Tanabe Pharma Corporation.
Research areas and target compounds involved in pharmaceutical * The MS instrument used in this poster is currently not for sale in the United States.
drug discovery is very wide ranging. Imaging mass spectrometry using
Mass Microscope is one powerful tool for the drug discovery
(pharmacokinetics, pharmacological mechanisms, toxicity), owing to
its ability to easily combine morphological observation from the optical
microscope and localization of target molecules from the mass
spectrometer image.

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