Page 18 - Shimadzu Journal vol.3 Issue3

Drug Discovery

Gentamicin Distribution in Rat Kidney
Abundance of gentamicin C 1 was higher in the renal cortex than in the renal medulla. The signal intensities of m/z 322 of gentamicin within the
kidney in distribution images corresponded well with the gentamicin concentrations.

Vehicle 3 mg/kg 10 mg/kg 30 mg/kg

Optical image

Strong

MALDI image
(m/z: 478.3 ˠ 322.2)
Pixel size: 50 µm
Weak

Optical image
(H&E stained)

Concentration
30.3 µg/g 93.6 µg/g 129.5 µg/g
(by LC-MS/MS)

MS/MS spectrum of gentamicin C 1 in rat kidney dosed 30 mg/kg of gentamicin

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