Page 11 - Shimadzu Journal vol.3 Issue3

Drug Discovery

Data analysis HZ-166

Peak area and % remaining values
Time Test compound Verapamil % remaining
(min) Peak area Peak area
0 815599 586830 99.98
10 702717 543057 87.53
20 693332 550156 84.60
30 655521 515666 80.23
40 608029 558567 76.24
50 604208 566352 74.73
60 561988 561427 69.06

5.0 5.0
ln (% peak area ratio) 4.6 HZ-166 ln (% peak area ratio) 4.8 CMD 45
4.8

4.6
4.4
4.4
4.2

4.0 4.2
0 10 20 30 40 50 60 70 0 10 20 30 40 50 60 70
Time (min) Time (min)

Metabolic parameters

t = 0.693 Intrinsic clearance = (V*0.693)
1/2
slope t
1/2
Percent remaining
V= incubation volume Metabolic rate = slope*[analyte]
[protein] [protein]

Compounds analyzed
Xhe-III-74 analogs
N O
N O
Percent remaining
N
N
HZ-166 CDM-45 Xhe-III-74
Chemical Formula: C 21 H 16 N 4 O 2
Molecular Weight: 356.38

HZ-166 analogs

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