Page 7 - Shimadzu LCMS-8045

Consistent High-Sensitivity Performance

Robust Ion Source and Sample Introduction System Newly Designed Ionization Unit

Shimadzu's MS interface includes a high-sensitivity heated ESI source, 12,000 plasma samples were injected over 25 days (over 480 samples per Designed without cables or tubes, removing the new ionization unit is simple: release a one-touch lever to open the unit and lift it out. In addition,
heated capillary sample introduction, and proprietary ion transfer optics. day). Picogram levels of alprazolam were analyzed with an RSD of 3.42%. no tools are needed to detach the needles fitted in APCI and DUIS units, allowing for easy maintenance.
These design features combine to produce robust MS performance.

2
5 ng/mL Alprazolam spiked in protein-crashed human plasma extracts (IS: 5 ng/mL alprazolam-d5)
m/z 309.15 > 205.1
1.5
Area ratio %RSD = 3.42%
Area ratio 1

480 injections/day
0.5 Column replaced after 7,500 injections
performed for 25 days

0
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 12000
Number of injections
LCMS-8045 Instrument Stability Test
×10 5 ×10 5 ×10 5 ×10 5
9.0 9.0 9.0 9.0
th
1 injection 5000 injection 10000 injection 12000 injection
st
th
th
6.0 6.0 6.0 6.0
3.0 3.0 3.0 3.0
0.0 0.0 0.0 0.0
0.8 0.9 1 1.1 1.2 1.3 1.4 0.8 0.9 1 1.1 1.2 1.3 1.4 0.8 0.9 1 1.1 1.2 1.3 1.4 0.8 0.9 1 1.1 1.2 1.3 1.4 Easy System Maintenance Reduces Downtime
min min min min
MRM Chromatograms of Alprazolam As with Shimadzu’s other triple quad systems, maintaining the LCMS-8045 is simple. Replacing the desolvation line (DL) and ESI capillary is quick and
easy. Additionally, the design allows users to replace the DL without breaking vacuum, providing greater uptime and usability.

Instrument Detection Limits: IDL Steps for DL Replacement
IDL is one measure of sensitivity for analytical instrumentation. The chromatograms below show the IDL for Reserpine and Chloramphenicol, obtained
using ESI and ESI , respectively. The IDL for Reserpine is 2.03 femtograms and 2.09 femtograms for Chloramphenicol. 2
−
+
1
(×100) (×100)
+
ESI (Reserpine) ESI (Chloramphenicol) 3
−
4.0
Mass on column 10 fg 1.50 Mass on column 10 fg DL (Desolvation Line)
3.5 IDL = 2.03 fg Area %RSD = 7.21 IDL = 2.09 fg Area %RSD = 7.40
n = 10 injections 1.25 n = 10 injections
3.0
Steps for ESI Capillary Replacement
2.5 1.00
2.0 0.75
1.5 2 fg on-column 3
0.50 2 fg on-column 1
1.0
0.25 2
0.5
0.0 0.00 ESI Capillary
0.0 0.1 0.2 0.3 0.4 min 0.00 0.25 0.50 min

LCMS-8045
6 Liquid Chromatograph Mass Spectrometer 7