Page 4 - Shimadzu LCMS-8045

High Performance for a Variety of Applications

Veterinary Drugs Water Quality Analysis

Nitrofuran metabolites in fish High-sensitivity analysis of 21 pharmaceuticals and personal care products in less than eight minutes
Nitrofurans are a class of synthetic antibiotics. The use of nitrofurans for results ranging from 0.05 to 20 ng/mL were obtained. The LCMS-8045 is Pharmaceutical and personal care products (PPCPs) encompass a family of become a significant threat to our water supply. The LCMS-8045 is
edible animal and fishery products is banned in many countries. The shown to be an excellent platform for routine high-sensitivity analysis in compounds used by individuals for health and cosmetic purposes. As capable of trace-level quantitation of many PPCPs without extensive
chromatogram below shows the recovery of nitrofuran metabolites from challenging matrices. populations increase, and the use of these products increases, PPCPs will sample preparation.
fish using the LCMS-8045. Excellent linearity and accurate quantitative
The limit of detection (LOD) and limit of quantitation (LOQ) for 21 PPCPs
(×10 5 ) (×10 5 )
1.50 AMOZ AMOZ 335.15 > 291.10(+) (×1.00) Calibration range, accuracy and limit of detection (LOD) 3.5 9 No. Compound LOD LOQ No. Compound LOD LOQ No. Compound LOD LOQ
SEM
209.05 > 166.10(+) (×20.0)
AHD 249.00 > 134.05(+) (×20.0) 18 (ng/mL) (ng/mL) (ng/mL) (ng/mL) (ng/mL) (ng/mL)
1.25 AOZ 236.10 > 134.00(+) (×5.00) Compound Calibration range Accuracy LOD 3.0 2 1 Lincomycin 0.01 0.04 8 Metoprolol 0.013 0.04 15 Clarithromycin 0.0034 0.01
SEM (ng/mL) (%) (ng/mL) 1 12 16
1.00 AHD 2.5 3 4 8 15 19 2 Trimethoprim 0.01 0.04 9 Dicorantil 0.005 0.015 16 Roxithromycin 0.0071 0.022
AMOZ 0.05 – 20 94.5 – 103.8 0.00024 2.0
0.75 AOZ 13 17 21 3 Pirenzepine 0.01 0.03 10 Sulfamethoxazole 0.003 0.01 17 Carbamazepine 0.01 0.03
SEM 0.05 – 20 93.2 – 111.6 0.015 1.5 5 7
0.50 6 10 20 4 Ofloxacin 0.01 0.03 11 Antipyrine 0.01 0.03 18 DEET 0.0062 0.018
AHD 0.05 – 20 95.5 – 102.1 0.001 1.0 11 5 Ciprofloxacin 0.002 0.006 12 Ifenprodil 0.0075 0.022 19 Crotamiton 0.005 0.015
0.25 14
AOZ 0.05 – 20 94.0 – 108.4 0.00096 0.5 6 Sulphapyridine 0.002 0.006 13 Erythromycin 0.015 0.045 20 Bezafibrate 0.0025 0.075
0.00
0.0 7 Carbazochrome 0.015 0.045 14 Azithromycin 0.01 0.03 21 Triclocarban 0.01 0.03
0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 min * Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 min
MRM chromatogram of nitrofuran metabolites * Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.
spiked in fish (0.5ng/mL) MRM chromatograms of 21 PPCPs (1.0 ng/mL) Limit of quantitation (LOQ) was calculated at S/N = 10.
Residual Pesticides Bioanalysis
Simultaneous analysis of residual pesticides in herbal supplements High-sensitivity quantitation of Amlodipine in plasma
Regulatory agencies worldwide provide oversight for an extensive and interferences, but the LCMS-8045 provides enough sensitivity to analyze Amlodipine is widely utilized for the treatment of high blood pressure and achieved. Both interday and intraday variabilities were assessed for quality
ever-increasing list of pesticides in order to promote food safety and most pesticides at the trace level of 10 parts per billion. Many pesticides angina. A high-sensitivity method for the quantitation of amlodipine in control samples. Percent RSD (Relative Standard Deviation) results were
protect public health. The MRM chromatogram below shows the methods also benefit from polarity switching, which the LCMS-8045 can human plasma using LCMS-8045 has been developed. Appropriate less than 5%.
simultaneous analysis of 153 pesticides spiked into Codonopsis pilosula at accomplish in just 5 msec. linearity ranging from 0.003 to 300 ng/mL with excellent accuracy was
0.01 mg/kg. Plant matrices can be very challenging due to background
Quantitative results of Amlodipine
(×10 6 ) 25 0.003 ng/mL – 300 ng/mL
4.5 r 2 > 0.9994 Lv Actual conc. Calculated Conc. Accuracy
4.0 20 11 levels (ng/mL) (ng/mL) (%)
3.5 1 0.003 0.003 104.5
3.0 15 2 0.01 0.01 97.2
2.5 Area ratio
2.0 10 3 0.03 0.031 103
1.5 4 0.1 0.107 107.1
1.0 5 5 0.3 0.268 89.4
0.5 6 1 1.062 106.2
0.0
0 7 3 2.958 98.6
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 min 0 50 100 150 200 250 300 Conc
8 10 10.293 102.9
153 regulated pesticides spiked into Codonopsis pilosula at 10 parts per billion Calibration curve of Amlodipine
9 30 27.37 91.2
10 100 98.722 98.7
Use Synchronized Survey Scan (SSS) to obtain both qualitative and (×10 6 ) Inten. 750 11 300 303.619 101.2
1.0 100 S/N = 10
quantitative information from a single injection. Full scan MS spectra will (Peak to peak) 0.003 ng/mL
2
(Matrix matched calibration sample)
be obtained when an MRM peak intensity threshold is exceeded. Interday and intraday variations of
0.002 mg/kg 0.5 50 500 Amlodipine spiked in human plasma
Concentration accuracy
0.0 0 QC sample %RSD (%)
Type Event# +/− Compound Name m/z Time (0.000 min – 15.939 min) 5.0 6.0 min 50 100 150 200 250 m/z (ng/mL)
MRM 63 + Metalaxy 280.10>220.20, 2 (×10 6 ) Inten. 250 Blank
Product Ion Scan 64 + > CE: −18.0, 50.00:300.00 100 Interday Low 0.1 3.78 97.8–109.1
MRM 65 + Atrazine 216.10>174.05, 2 4.0
Product Ion Scan 66 + > CE: −20.0, 50.00:250.00 Variation Medium 5 3.16 90.0–95.4
MRM 67 + Fensulfothoin-sulfone 324 3.0 (n = 6)
Product Ion Scan 68 + > CE: −28.0, 50.00:350.00 High 240 0.85 95.9–97.6
MRM 69 + N-desethly-pirimiphos-me 0.01 mg/kg 2.0 50 0.0
Product Ion Scan 70 + > CE: −25.0, 50.00:300.00 min Intraday Low 0.1 4.5 87.5–110.3
1.0 1.0 1.5 2.0 Variation Medium 5 4.92 88.1–102.9
Method settings and Synchronized Survey Scan results for Metalaxyl MRM chromatogram of Amlodipine at the lower limit of quantitation
0.0 0 (n = 18) High 240 4.25 88.3–104.0
5.0 6.0 min 50 100 150 200 250 m/z Black: Blank, Pink: Matrix matched calibration sample (0.003 ng/mL)
LCMS-8045
4 Liquid Chromatograph Mass Spectrometer 5