Page 9 - Shimadzu UV-1280

Data Storage on USB Flash Drives Applications

Pharmaceuticals and Life Sciences

The UV-1280 includes programs for enzymatic reaction measurements, and DNA/protein quantitation, essential in
bioscience/life science fields. A monitor double-beam has been adopted using a highly stable deuterium lamp and halogen
lamp, which is optimal for kinetics measurements tracking changes over time. With a wealth of accessories, this instrument can
even accommodate trace samples and measurements as is in test tubes.

DNA/Protein Quantitation

Program: DNA/Protein Quantitation
Easily obtain DNA and protein concentrations, which are measured directly from absorption
bands in the UV wavelength region, without performing coloring operations. With preset
wavelengths and computational formulas, simply position the sample and press the
Using USB flash drives makes it easy to transport analysis data START/STOP key for one-touch quantitation results. The measurement wavelengths and
computational coefficients can be freely changed.
and to store large amounts of data in a PC. Calculates two-wavelength absorbance ratios and DNA/protein concentrations.
Select from the following two quantitation formulas*:
USB flash drives can be connected directly to the UV-1280.
*Absorbance at 320 nm can be used for background correction.
Data for spectra and time-course curves can be displayed and saved with 1) A1 = 260 nm absorbance; A2 = 230 nm absorbance
commercial spreadsheet software. Absorbance ratio = A1/A2
DNA concentration = 49.1 × A1 - 3.48 × A2
Protein concentration = 183.0 × A2 - 75.8 × A1
UV-1280 + USB flash drive + PC 2) A1 = 260 nm absorbance; A2 = 280 nm absorbance
Absorbance ratio = A1/A2 References
With the UV-1280, the curve-related data (spectra and time-course DNA concentration = 62.9 × A1 - 36.0 × A2 1.Warburg and Christian, (1942) Biochem. Z. 310, 384-421.
curves) can be converted to, and saved in, Protein concentration = 1552.0 × A2 - 757.3 × A1 2.Kalb and Bernlohr, (1977) Anal. Biochem. 82, 362-371.
CSV format. Transferring this data with USB flash drives allows it to
be read directly at a PC using commercial spreadsheet software.
Trace Sample Measurements
Analysis Compatibility Table Spectral measurements of a 100 µL of dsDNA sample were performed using a supermicro cell
holder. A conventional square cell with a path length of 10 mm requires approx. 3.5 mL of
sample. However, a supermicro cell allows measurements with a 100 µL to 200 µL sample.
Pharmaceuticals and Life Sciences Foods
Program: Spectrum
Accessories: supermicro cell, supermicro cell holder, and sample compartment unit
DNA/protein quantitation Optimal Pigment measurements Optimal
Culture fluid turbidity measurements Optimal Quantitation of vitamins, food additives, and minerals Optimal
Enzymatic reaction measurements Optimal

Environmental Supermicro Cell Holder Supermicro Cell
Chemicals
Culture Fluid Turbidity Measurements
Turbidity measurements Optimal
Transmittance measurements for films Optimal Quantitation of total phosphorus and total Optimal If the photometric function is used, dilution and other coefficients
nitrogen in river water, and lakes and marshes can be calculated automatically. In addition, measure microbial
Thickness measurements for thin films and other films Suboptimal counts as is in test tubes using the test tube holder (special order
Measurements of plating liquids
(hexavalent chromium, aluminum, nickel, etc.) Optimal product).
Program: Photometric
Quantitation of iron, copper, arsenic, and Optimal Accessory: Test tube holder
ammonia in water The sample compartment unit (P/N 206-60184-07) is separately required. Test Tube Holder

UV-1280
8 UV-VIS Spectrophotometer 9