Page 10 - Shimadzu UV-1280

Applications

Chemicals Environmental

A wealth of accessories enables analysis of a variety of samples, including liquids and films. In addition, the UV-1280 can Easily perform both transmitted light turbidity measurements and RoHS hexavalent chromium quantitation with a simple
accommodate both spectral and quantitation measurements. quantitation program. In addition to the standard sample chamber for 10-mm cells, you can use a long path length cell for
measuring low concentration solutions, or a sipper unit that does not require a cell.

Transmittance Measurements of Films Transmitted Light Turbidity Measurement as per JIS K0101
"Testing Methods for Industrial Water"
This is an example of a spectral measurement of colored cellophane.
Absorption by the red colored cellophane is evident at around 530 nm, and by the blue
colored cellophane at around 650 nm.
Program: Spectrum
Accessories: sample compartment unit and film holder

Film Holder

In accordance with JIS K0101, a standard formazin solution was prepared. The absorbance at 660 nm Abs = K1C + K0
K1 = 1.5908e-03
was measured, and a calibration curve was created. A rectangular cell with a 50 mm path length is used K0 = 1.0420e-02
Foods 2
for turbidities of 4 to 80 degrees, and a square cell with a 10 mm path length for 20 to 400 degrees. r = 0.9996
Perform single wavelength measurements with a single touch of a button. Coefficients can also be calculated simultaneously. Program: Quantitation
Accessories: Sample compartment unit, long-path rectangular cell holder, and a 50-mm rectangular cell
The data is exported to a CSV file, simplifying subsequent analysis. In addition to quantitation using one or two wavelengths,
a multi-component quantitation function using multiple peaks is also included as standard.

Color Value Measurements of Food Dyes

1.000 Dye concentrations (color values) were determined by measuring the absorbance.
Conventionally, the color value is determined by measuring the absorbance at the wavelength
0.800
Absorbance 0.600 Yellow Dye #4 5ppm of maximum absorption in the visible range in the solution with the food coloring, and then
Blue Dye #1
Blue Dye #1
5ppm
ellow Dye #4
Y
converting the value to the absorbance for a 10 w/v% solution (E10 % 1 cm).
10 ppm
10 ppm
Red Dye #2
Red Dye #2
10ppm
0.400 10ppm Program: Spectrum
Accessory: 10-mm standard cell
0.200
Measurement Results
0.000
380.0 500.0 600.0 700.0 780.0
Wavelength (nm) Sample Name Collected Material (g) F Cell Used λmax ABS Color Value
Absorption Spectra for Dyes Blue Dye #1 0.500 1000 STDCELL 629.5 0.7488 14976
Red Dye #2 0.100 100 STDCELL 521.5 0.3889 3889
Yellow Dye #4 0.100 100 STDCELL 426.5 0.4611 4611
10×A×F
Color Value=
Quantity of sample collected (g)
F: Dilution rate to adjust the measured absorbance so that it falls in a range between 0.3 and 0.7
A: Measured absorbance
UV-1280
10 UV-VIS Spectrophotometer 11