Page 7 - Shimadzu UV-1280

A Variety of High-Level Measurement Modes

Equipped with a range of programs, the UV-1280 can be used for everything from photometric, spectral, and kinetics 4 Kinetics Mode
measurements to DNA/protein and high-level multi-component quantitation.
Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method automatically
calculates the amount of change per minute, and then calculates an activity value from a specified coefficient. The rate measurement method,
which determines whether the absorbance is changing linearly, can also be selected. In addition, add the CPS-100 thermoelectrically
1 Photometric Mode temperature-controlled cell positioner for measurement of multiple samples in succession.

Measures the absorbance or transmittance at a single wavelength or multiple (up to eight) wavelengths. The instrument is also capable of
simple quantitation using the K-factor method. For a multiple-wavelength measurement, calculations can be performed on the data obtained
for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.

5 Time Scan Mode

Measures the change in absorbance, transmittance or energy as a function of time. Add the CPS-100 thermoelectrically temperature-controlled
cell positioner for simultaneous measurement of multiple samples under constant-temperature conditions.
2 Spectrum Mode
6 Multi-Component Quantitation Mode
A sample spectrum is recorded using wavelength scanning. Repeat
scans let you follow sample changes over time. Zoom in on the
Quantitates up to eight components mixed in a single sample.
finished spectrum for a better view; subsequently, use the peak/valley
The calibration equation is determined using pure or mixed
pick function to select maxima and minima and perform a wide
components with known values.
variety of data processing functions.

3 Quantitation Mode

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Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns. Allows various Biomethod Mode
combinations of wavelength number (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order).
Determine DNA and protein concentrations with the following
quantitation methods using the bioscience/life science program
included as standard.
DNA/Protein Quantitation
・Quantitates DNA or protein using the absorbance at 260/230 nm or
260/280 nm.
Protein Quantitation
• Lowry method
• BCA method (method using bicinchoninic acid)
• Biuret method
• CBB method (method using Coomassie Brilliant Blue G-250)
• UV absorption method (direct measurement at 280 nm)

UV-1280
6 UV-VIS Spectrophotometer 7