Page 8 - Shimadzu SPM-8100FM

Examples of Hydration/Solvation Structure Measurements The Principle of FM-Type AFM

Interface Structure of 1-Decanol Contact with Graphite The frequency of a vibrating cantilever is measured in dynamic mode, and interactions with the sample are detected.
Specifically, the cantilever is moved in a non-contact state, so that the cantilever frequency shift ( f ) is constant.
The molecular film of 1-decanol over graphite was observed (figure at left). The joining of two molecules of decanol and molecular
This enables highly sensitive force detection, 20 times better than with existing methods, thereby improving the
film formation can be observed. The cross-section structure of decanol liquid contact with an adsorbed molecular layer was measured
image resolution.
(figure at right). It was found that the decanol organized in a layer structure and there existed inhomogeneous distribution in an
in-plane direction.
Cantilever resonance curve

f
Probe Cantilever
(Dynamic resonance system)
Sample
Cantilever Oscillation amplitude
6 nm
Interaction force
(attraction)
Non contact

Frequency
40 nm
Z
References
X
Cited References
1) Ryohei Kokawa, Masahiro Ohta, Akira Sasahara, Hiroshi Onishi, Kelvin Probe Force Microscopy Study of a Pt/TiO 2 Catalyst Model Placed in an Atmospheric Pressure of N 2
Environment, Chemistry – An Asian Journal, 7, 1251-1255 (2012).
A Saturated Aqueous Solution in Contact with p-nitroaniline Crystals 6) 2) K. Nagashima, M. Abe, S. Morita, N. Oyabu, K. Kobayashi, H. Yamada, R. Murai, H. Adachi, K. Takano, H. Matsumura, S. Murakami, T. Inoue, Y. Mori, M. Ohta, R. Kokawa, Molecular
resolution investigation of tetragonal lysozyme(110) face in liquid by FM-AFM, Journal of Vacuum Science and Technology B 28 (2010) C4C11-C4C14
In the Z-X in measurements on the left, the convex area is the position of the benzene ring, and the concave area is the position of 3) Sebastian Rode, Noriaki Oyabu, Kei Kobayashi, Hirofumi Yamada, and Angelika Kuhnle, True Atomic-Resolution Imaging of (1014) Calcite in Aqueous Solution by Frequency
the hydrophilic functional group. From the force curves at each position (Z- f curves), it is evident that there is strong hydration due Modulation Atomic Force Microscopy, Langmuir, 2009, 25 (5), pp 2850–2853
4) K. Kimura, S. Ido, N. Oyabu, K. Kobayashi, Y. Hirata, T. Imai, H. Yamada, Visualizing water molecule distribution by atomic force microscopy, Journal of Chemical Physics, 132, 19,
to the localization of water molecules in the concave area representing the hydrophilic group. This data suggests that the structure is
194705 (2010).
stabilized by hydrogen bonding of the water molecules with the polar groups, as shown by the model in the figure at the bottom. 5) Kei Kobayashi, Noriaki Oyabu, Kenjiro Kimura, Shinichiro Ido, Kazuhiro Suzuki, Takashi Imai, Katsunori Tagami, Masaru Tsukada and Hirofumi Yamada, Visualization of hydration
layers on muscovite mica in aqueous solution by frequency-modulation atomic force microscopy, Journal of Chemical Physics, 138, 184704 (2013).
6) Rina Nishioka, Takumi Hiasa, Kenjiro Kimura, and Hiroshi Onishi, Specific Hydration on p Nitroaniline Crystal Studied by Atomic Force Microscopy, J. Phys. Chem. C, 117, 2939−
2943 (2013).
+300 Hz Instrument References
Frequency shift • Kei Kobayashi, Hirofumi Yamada, and Kazumi Matsushige, Reduction of frequency noise and frequency shift by phase shifting elements in frequency modulation

• Ryohei Kokawa and Masahiro Ohta, Development of a High Resolution FM-AFM Working in Air or Solution, Microscopy, Vol. 47, No. 1 (2012) (Japanese)

atomic force microscopy, Rev. Sci. Instrum., 82, 033702 (2011).

Application Examples
-40 Hz
0 0.5 1.0 1.5 2.0 • Shinichiro Ido, Kenjiro Kimura, Noriaki Oyabu, Kei Kobayashi, Masaru Tsukada, Kazumi Matsushige and Hirofumi Yamada, Beyond the Helix Pitch: Direct
Relative distance / nm Visualization of Native DNA in Aqueous Solution, ACS Nano, 7, 1817-1822 (2013).
Z-X Measurement of p-nitroaniline interface Force curve • Takumi Hiasa and Hiroshi Onishi, Competitive Adsorption on Graphite Investigated Using Frequency-Modulation Atomic Force Microscopy: Interfacial Liquid
Water molecules Structure Controlled by the Competition of Adsorbed Species, Langmuir, 29, 5801-5805 (2013).

This Instrument has been commercially developed through collaboration with the Yamada group at Kyoto University, the Morita group at Osaka University, the Onishi group at
Kobe University, the Tomitori group at JAIST, and the Arai group at Kanazawa University.
All data on this page was obtained using the petri dish type solution cell (option).

SPM-8100FM
8 High Resolution Scanning Probe Microscope 9