Page 9 - Shimadzu Nexera UC - Nexera UC Prep

Analysis

Add fractionation capabilities to the analytical SFC system System configuration examples

Upgrade to an analytical fraction system SFC-UV / SFC-MS System

By adding a fraction collector to the analytical scale SFC, small-volume fractionation is possible. A complete This is the minimum setup of Nexera UC and can replace both normal
workflow, from method development to fractionation, can be carried out seamlessly on one system. phase and reverse phase HPLCs. A wide range of analyte polarity can
be covered by the combination of supercritical CO2 and modifiers (for
example, MeOH). Hazardous organic solvents such as hexane or
chloroform are eliminated. In addition, the system reduces
environmental impact by utilizing low-toxicity mobile phases and
completing analyses in a shorter time. Both UV-Vis and MS detectors
such as the triple quad LCMS-8050 can be successfully coupled with
this SFC system.

Fraction collector
FRC-40 SF
Nexera UC
Chiral Screening System

This system is best for developing methods to separate chiral
compounds. It automatically generates a large number of
methods by utilizing combinations of up to 12 columns, four
modifiers, and a different ratio of modifiers to mobile phase.
Small-volume fractionation is possible

The patented gas–liquid separator, the LotusStream successfully reduces sample dispersion due to the
™
volumetric expansion of CO2. It allows fractionation of small volumes, even into vessels such as 1.5 mL vials.
The optical isomers of linalool were fractionated with high purity.
mAU

(+)-Linalool
(−)-Linalool
Nexera UC/s UHPLC / SFC Switching System

This system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 min sample in each separation mode. It enhances user-friendliness and operability by allowing the investigation of
Preparative chromatogram of linalool separation conditions and performing reverse-phase high-speed analysis in a single system. Shimadzu also
provides a kit to upgrade from your current UHPLC system to the UHPLC/SFC switching system.
mAU

(+)-Linalool
(−)-Linalool

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 min
Reanalysis chromatogram of the recovered solution

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