Page 7 - Shimadzu Nexera UC - Nexera UC Prep

Analysis

Ensures high-throughput method development and scaling up

Automatically performs a variety of method scouting processes Method scouting for optimization of separation conditions and scaling up
to preparative size
The high-speed performance of SFC can shorten the time required for method scouting. The system automatically
generates a large number of methods by utilizing combinations of up to 12 columns, four modifiers, and different
For high-purity isolation, target peaks need to be adequately separated, for which the user must determine
ratios of modifiers to mobile phase. With the UHPLC / SFC Switching system, screening by UHPLC and SFC can be
optimum column and separation parameter settings (method scouting). The Nexera UC chiral screening system and
carried out in the same batch. Moreover, Method Scouting Solution, optional software for method scouting, makes it
Method Scouting Solution can be used to screen columns more quickly and accurately (Step 1). Once the optimum
easy to set up multiple conditions.
column has been identified, smoothly scale up to preparative scale flow rates, preserving the peak separation
while increasing the mass load (Step 2).
*Preparative SFC: page 10 and following.
Step 1 Method scouting at analytical scale

Method scouting is easy even for first-time users—simply execute the batch table generated automatically by Method
Scouting Solution. The system can automatically switch between different settings to run the scouting process continuously
day or night, even for multiple modifiers and columns. Various types of data can be displayed in the data browser and
multi-data reports generated with resolution values for all data to assist in evaluating separation conditions.
Step 2 Scaling up 4.6 mm I.D. column (Nexera UC)
mAU (×1,000)
Column: Shim-pack UC-PBr 4.6 × 250 mm
Using a Shim-pack UC series column enables you Flow rate: 3.0 mL/min
to increase mass load while maintaining Injection volume: 20 µL
separation performance. The optimum Fraction volume: 10 mg
preparative column determined from Step 1 can
be used to scale up the column size, flow rate
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
and injection volume based on the desired
fraction volumes.
20 mm I.D. column (Nexera UC Prep)
mAU (×1,000)
A screen shot of Method Scouting Solution for Nexera UC user interface. Column: Shim-pack UC-PBr 20 × 250 mm
Flow rate: 56.7 mL/min
Injection volume: 500 µL
Fraction volume: 250 mg
Chiral analysis with “Nexera UC Chiral Screening System”

CHIRALPAK Series and CHIRALCEL Series columns (Daicel Corporation) for chiral analysis are capable of resolving 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
®
®
a wide variety of compounds by showing complementary separation targets. The combination of the Nexera UC
Chiral Screening System and these columns simplifies method scouting for chiral analysis. Selecting an SFC Column
The HPLC mobile phase for reverse phase analysis is very different from the one used for normal phase analysis. In contrast,
SFC uses a mixture of supercritical CO2 and a modifier (an organic solvent such as methanol) regardless of the stationary phase
mAU
used. Therefore, the same mobile phase composition can be used for serial analysis through all columns. Column scouting is
300 Indapamide
RT = 2.644 effective by using the following set of six columns, each providing a different separation selectivity.
Rs = 2.66
200
6 columns set
UC-ODS UC-Sil II UC-Diol II UC-PolyVP UC-PolyBT UC-PBr
100
Br Br
OH
0 OH Br
Br
Chemistry O O Br
0.0 1.0 2.0 3.0 4.0 5.0 min
Si Si
Columns: CHIRALPAK AD-3
®
Modifier: Acetonitrile/Ethanol=7/3 OH
The separation mode is This is excellent for retention The separation mode is A favorable peak shape This is excellent for With ODS, separation of
reverse phase. Retention of basic compounds and normal phase. This is obtained even resolving aromatic poorly retained
Features
is provided through recognition of their tertiary inhibits non-specific without acid-base compounds through π-π compounds is improved.
hydrophobic interaction. structures. interactions. additives. interactions.
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