Page 6 - Shimadzu LCMS-IT-TOF

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High-Speed and High-Accuracy MS Measurement

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The LCMS-IT-TOF is intended to strongly assist in the identification of target compounds by using high-speed/high-accuracy MS data in
R&D fields such as impurity analysis, metabolic profiling and biomarker research.

HO
HO HO HO
N
N N N O
O O O
OH OH OH H3CO
O O HO HO
(× 10,000,000) H3C
1
MS scan points
Naloxone
1.00
Naltrexone Half Height Peak
Width: 1.2 sec
6-beta-naltrexol
Buprenorphine
0.50
2.4 4.8 7.2 sec
1 min+
0.00
0.0 0.5 1.0 1.5 2.0 min

MS 1 [M+H] + HO 342.1691
Expected 342.1705
O N 4.09 ppm
OH
O
C20H23NO4

100 150 200 250 300 350 400 450 m/z
MS 2 [342-H2O] + 324.1605
Expected 324.1600
-1.54 ppm

100 150 200 250 300 350 400 450 m/z
MS 3 [324-C4H6] + 270.1137
270.1137
Expected 270.1130
-2.59 ppm
-2.59 ppm

100 150 200 250 300 350 400 450 m/z
External Standard Method

The data above shows the analysis of Column : Shim-pack XR-ODS (2.0 × 30 mm, 2.2 μm)
four compounds within 2.0 minutes. Mobile phase A : 0.1 % formic acid
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The Auto MS function makes it possi- Mobile phase B : 0.1 % formic acid in methanol
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ble to obtain highly accurate MS data
Time program : 4 % B (0 min) – 100 % B (0.5 min) – 4 % B (0.51– 2.00 min)
via an external standard, taking ad-
vantage of high-speed mass spectrum Flow speed : 0.5 mL/min
measurement performance. Injection volume : 5 μL (1 ng/μL)
Column oven temperature : 50°C

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