Page 10 - Shimadzu LCMS-IT-TOF

n
Intelligent Auto MS Function

n
What is the Auto MS Function?
Samples cannot be recaptured once injected for LC/MS analysis; TIC
therefore, it is vital that instruments can automatically select the
appropriate precursor ions. With the LCMS-IT-TOF, a variety of pre-
cursor ion selection criteria, such as the selection of ions in order of
intensity or m/z is available, in addition to intelligent automatic pre-
cursor selection, such as a monoisotopic peak selector and charge-
state filtering.
MS 1 MS 1
Threshold Threshold
Main Functions for Precursor Ion Selection
Selection in the order of intensity or m/z
m/z m/z
Charge state filter
Selecting precursor ions automatically
Monoisotopic filter and measuring MS 2
Exclusion ion (Automatic exclusion)
MS 2
Pair peak selection
Preference Ion Selection
Neutral Loss Survey
m/z

Principles of Neutral Loss Survey

3
MS measurements are automatically performed if the specified tailed information about target ions is obtained using the neutral
2
neutral loss is observed in the MS spectrum. With the Neutral Loss loss survey function, it can become a powerful tool for supporting
3
Survey, only the target ions are measured in MS , enabling one to the identification of compounds (e.g., phase II metabolites for drug
obtain desired information effectively without loss of time. As de- discovery research).
Judgment of neutral loss
MS 2 [Ma-m] + MS 3
Ma + [Ma-m] +
Setting neutral loss m
m
MS 1 Ma +
Mb + m/z m/z
+
+
MS 2 measurement with Ma MS 3 measurement with [Ma-m]
as precursor ion as precursor ion
+
MS 2 [Mb-n]
m/z Mb +
Not going on MS measurement
3
n
m/z
MS measurement with Mb +
2
as precursor ion

10