Page 7 - Shimadzu LCMS-8060NX

Robustness and Ease of Use

Maximized sensitivity, minimized matrix effects New AI-derived parameters optimized for a wide range of compounds

In the newly-developed IonFocus unit, the focus electrodes reduce sensitivity loss from matrix effects by expelling contaminants with The LCMS-8060NX uses new default parameters optimized for a wide range of compounds based on research with AI (Artificial
greater efficiency. The contaminants below show an analysis example of residual pesticides in crops. Using the LCMS-8060NX's Intelligence) processes, enabling excellent results without the need for labor-intensive investigation of analysis conditions. As shown
world-class polarity reversal speed (5 ms) and data capture speed (1.5 ms), stable data was obtained even for high-speed analysis of below, this boosts the signal intensity for a variety of target compounds to an average of 2.6 times the intensity achieved with
multiple components. The IonFocus unit also introduces ions into the mass spectrometer more efficiently, improving the signal previous parameters.
intensity. Of the 100 compounds tested, 96% showed excellent recoveries.
Hydrophilic compounds Lipids Pesticides
(×10 5 ) (×10 4 ) (×10 6 )
9.0 2.00 With IonFocus (×10 7 ) (×10 6 ) (×10 5 )
9.0 Positive Negative Positive 1.5 2.00
8.0 m/z 312.05>267.05 8.0 m/z 212.90>140.95 m/z 414.25>183.20 Without IonFocus 1.4 Positive Positive 8.5 Positive
7.0 S:×1.5 7.0 S:×1.5 1.50 S:×2.2 1.3 m/z 116.10>70.15 1.75 m/z 348.20>62.10 8.0 m/z 255.00>208.90
7.5
6.0 6.0 1.2 7.0
5.0 5.0 1.00 1.1 1.50 6.5
6.0
4.0 4.0 1.0 S:×2.4 1.25 S:×2.6 5.5 S:×3.2
3.0 3.0 0.9 5.0
2.0 2.0 0.50 0.8 1.00 4.5
0.7
1.0 1.0 0.6 0.75 4.0
3.5
0.0 0.0 0.5 3.0
0.00
3.75 4.00 4.25 4.50 4.75 5.00 min 3.75 4.00 4.25 4.50 4.75 5.00 min 5.25 5.50 5.75 6.00 6.25 6.50 min 0.4 0.50 2.5
2.0
0.3
Imazaquin Mecoprop (MCPP) Cinosulfuron 0.2 0.25 1.5
(×10 7 ) 0.1 1.0
1 compound 3 compounds 0.5
0.0 0.00 0.0
1.00 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min 17.0 17.5 18.0 18.5 19.0 19.5 min 3.00 3.25 3.50 3.75 4.00 min
Proline Anandamide Fluroxypyr
(×10 5 ) (×10 4 ) (×10 4 )
0.75 3.75 2.00 4.00
3.50 Negative Negative Negative
3.25 m/z 167.10>123.95 1.75 m/z 319.20>155.10 3.50 m/z 199.25>127.10
0.50 Recovery rate 3.00
0 - 70% 2.75 1.50 3.00
0.25 96 compounds 70 - 120% 2.50 S:×2.8 1.25 S:×2.4 2.50
2.25
120 - 150% 2.00 1.00 S:×2.2
1.75 2.00
0.00
1.50 0.75 1.50
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 min Excellent recovery rate among 100 pesticides 1.25 0.50
1.00
MRM chromatogram of 100 pesticides (0.02ppm) in crops 0.75 1.00
0.50 0.25 0.50
0.25 0.00 0.00
Superior robustness 0.00 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min 18.00 18.25 18.50 18.75 19.00 min 3.25 3.50 3.75 4.00 4.25 4.50 min
Uric acid 8,9-EET (8,9-epoxyeicosatrienoic acid) Cloprop
The excellent robustness of the LCMS-8060NX can be seen most clearly during consecutive analyses of samples of biological origin
that tend to contaminate instruments. We performed consecutive analyses of human blood plasma spiked with alprazolam on the New parameters Conventional parameters
LCMS-8060NX and plotted the resulting area values and area ratios (internal standard material: alprazolam-d5). To evaluate the
robustness under even more demanding conditions, we performed a total of 15,000 consecutive analyses without using valves to Efficient desolvation for higher efficiency
remove impurities. As shown in the data below, the results were extremely stable, with an area value reproducibility of 1.79 %RSD
and an area ratio reproducibility of 1.71 %RSD. This demonstrates the excellent stability of results with the LCMS-8060NX even A new heat-assisted design improves the desolvation efficiency and dramatically enhances the sensitivity for challenging molecules
during consecutive analyses of samples with large amounts of contaminants such as urine and blood plasma. such as steroid hormones.
x 10 5 (x10 6 ) (x10 6 )
6.0 1.4 Positive 1.2 Positive
Alprazolam (Internal Standard : Alprazolam-d5) 2.50 m/z 1.1 m/z
m/z 308.95 > 281.00 270.90>213.10 291.20>255.15
5.0 1.2 2.25 1.0
ESI heater:
1.0 Improved heat transfer 2.00 S:×3.3 0.9 S:×2.0
4.0 efficiency 1.75 0.8
Peak area 3.0 Area ratio 1.71 %RSD 0.8 Area ratio 1.50 0.6
0.7
1.25
0.6
2.0 Heating gas: 1.00 0.5
0.4
Peak area 1.79 %RSD 0.4 Increased maximum 0.75 0.3
1.0 0.2 flow rate 0.50 0.2
0.25 0.1
0.0 0.0
0 5,000 10,000 15,000 0.00 0.0
Injection number 0.7 0.8 0.9 1.0 1.1 1.2 min 1.00 1.25 1.50 min
Dehydroepiandrosterone 5α-Dihydrotestosterone
Results from Consecutive Analyses of Alprazolam-Spiked Human Blood Plasma LCMS-8060NX LCMS-8060
LCMS-8060NX
6 Liquid Chromatograph Mass Spectrometer 7