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Robustness and Ease of Use






            Maximized sensitivity, minimized matrix effects                                                                           New AI-derived parameters optimized for a wide range of compounds

            In the newly-developed IonFocus unit, the focus electrodes reduce sensitivity loss from matrix effects by expelling contaminants with   The LCMS-8060NX uses new default parameters optimized for a wide range of compounds based on research with AI (Artificial
            greater efficiency. The contaminants below show an analysis example of residual pesticides in crops. Using the LCMS-8060NX's   Intelligence) processes, enabling excellent results without the need for labor-intensive investigation of analysis conditions. As shown
            world-class polarity reversal speed (5 ms) and data capture speed (1.5 ms), stable data was obtained even for high-speed analysis of   below, this boosts the signal intensity for a variety of target compounds to an average of 2.6 times the intensity achieved with
            multiple components. The IonFocus unit also introduces ions into the mass spectrometer more efficiently, improving the signal   previous parameters.
            intensity. Of the 100 compounds tested, 96% showed excellent recoveries.
                                                                                                                                              Hydrophilic compounds                   Lipids                           Pesticides
                        (×10 5 )              (×10 4 )               (×10 6 )
                                            9.0                   2.00                      With IonFocus                                    (×10 7 )                         (×10 6 )                          (×10 5 )
                      9.0  Positive            Negative               Positive                                                             1.5                              2.00
                      8.0  m/z 312.05>267.05  8.0  m/z 212.90>140.95  m/z 414.25>183.20     Without IonFocus                               1.4  Positive                        Positive                      8.5  Positive
                      7.0          S:×1.5   7.0          S:×1.5   1.50          S:×2.2                                                     1.3  m/z 116.10>70.15            1.75  m/z 348.20>62.10            8.0  m/z 255.00>208.90
                                                                                                                                                                                                              7.5
                      6.0                   6.0                                                                                            1.2                                                                7.0
                      5.0                   5.0                   1.00                                                                     1.1                              1.50                              6.5
                                                                                                                                                                                                              6.0
                      4.0                   4.0                                                                                            1.0        S:×2.4                1.25          S:×2.6              5.5           S:×3.2
                      3.0                   3.0                                                                                            0.9                                                                5.0
                      2.0                   2.0                   0.50                                                                     0.8                              1.00                              4.5
                                                                                                                                           0.7
                      1.0                   1.0                                                                                            0.6                              0.75                              4.0
                                                                                                                                                                                                              3.5
                      0.0                   0.0                                                                                            0.5                                                                3.0
                                                                  0.00
                       3.75 4.00 4.25 4.50 4.75 5.00 min  3.75 4.00 4.25 4.50 4.75 5.00 min  5.25 5.50 5.75 6.00 6.25 6.50 min             0.4                              0.50                              2.5
                                                                                                                                                                                                              2.0
                                                                                                                                           0.3
                             Imazaquin            Mecoprop (MCPP)         Cinosulfuron                                                     0.2                              0.25                              1.5
               (×10 7 )                                                                                                                    0.1                                                                1.0
                                                                                  1 compound  3 compounds                                                                                                     0.5
                                                                                                                                           0.0                              0.00                              0.0
            1.00                                                                                                                              1.0  1.5  2.0  2.5  3.0  3.5  4.0 min  17.0  17.5  18.0  18.5  19.0  19.5  min  3.00  3.25  3.50  3.75  4.00  min
                                                                                                                                                    Proline                         Anandamide                         Fluroxypyr
                                                                                                                                             (×10 5 )                         (×10 4 )                          (×10 4 )
            0.75                                                                                                                          3.75                              2.00                              4.00
                                                                                                                                          3.50  Negative                        Negative                         Negative
                                                                                                                                          3.25  m/z 167.10>123.95           1.75  m/z 319.20>155.10           3.50  m/z 199.25>127.10
            0.50                                                                                     Recovery rate                        3.00
                                                                                                       0 - 70%                            2.75                              1.50                              3.00
            0.25                                                                     96 compounds      70 - 120%                          2.50           S:×2.8             1.25            S:×2.4            2.50
                                                                                                                                          2.25
                                                                                                       120 - 150%                         2.00                              1.00                                            S:×2.2
                                                                                                                                          1.75                                                                2.00
            0.00
                                                                                                                                          1.50                              0.75                              1.50
                3.5  4.0  4.5  5.0  5.5  6.0  6.5  7.0  7.5  8.0  8.5  9.0  9.5  10.0 min  Excellent recovery rate among 100 pesticides   1.25                              0.50
                                                                                                                                          1.00
                          MRM chromatogram of 100 pesticides (0.02ppm) in crops                                                           0.75                                                                1.00
                                                                                                                                          0.50                              0.25                              0.50
                                                                                                                                          0.25                              0.00                              0.00
            Superior robustness                                                                                                           0.00  1.0  1.5  2.0  2.5  3.0  3.5  4.0 min  18.00  18.25  18.50  18.75  19.00  min  3.25  3.50  3.75  4.00  4.25  4.50 min
                                                                                                                                                   Uric acid                 8,9-EET (8,9-epoxyeicosatrienoic acid)     Cloprop
            The excellent robustness of the LCMS-8060NX can be seen most clearly during consecutive analyses of samples of biological origin
            that tend to contaminate instruments. We performed consecutive analyses of human blood plasma spiked with alprazolam on the                                     New parameters      Conventional parameters
            LCMS-8060NX and plotted the resulting area values and area ratios (internal standard material: alprazolam-d5). To evaluate the
            robustness under even more demanding conditions, we performed a total of 15,000 consecutive analyses without using valves to   Efficient desolvation for higher efficiency
            remove impurities. As shown in the data below, the results were extremely stable, with an area value reproducibility of 1.79 %RSD
            and an area ratio reproducibility of 1.71 %RSD. This demonstrates the excellent stability of results with the LCMS-8060NX even   A new heat-assisted design improves the desolvation efficiency and dramatically enhances the sensitivity for challenging molecules
            during consecutive analyses of samples with large amounts of contaminants such as urine and blood plasma.                 such as steroid hormones.
                                         x 10 5                                                                                                                                               (x10 6 )              (x10 6 )
                                      6.0                                           1.4                                                                                                        Positive           1.2  Positive
                                         Alprazolam (Internal Standard : Alprazolam-d5)                                                                                                    2.50  m/z              1.1  m/z
                                         m/z 308.95 > 281.00                                                                                                                                   270.90>213.10         291.20>255.15
                                      5.0                                           1.2                                                                                                    2.25                   1.0
                                                                                                                                                                    ESI heater:
                                                                                    1.0                                                                             Improved heat transfer  2.00        S:×3.3    0.9        S:×2.0
                                      4.0                                                                                                                           efficiency              1.75                   0.8
                                    Peak area  3.0      Area ratio 1.71 %RSD        0.8  Area ratio                                                                                        1.50                   0.6
                                                                                                                                                                                                                  0.7
                                                                                                                                                                                           1.25
                                                                                    0.6
                                      2.0                                                                                                                           Heating gas:           1.00                   0.5
                                                                                                                                                                                                                  0.4
                                                        Peak area 1.79 %RSD         0.4                                                                             Increased maximum      0.75                   0.3
                                      1.0                                           0.2                                                                             flow rate               0.50                   0.2
                                                                                                                                                                                           0.25                   0.1
                                      0.0                                           0.0
                                        0            5,000         10,000        15,000                                                                                                    0.00                   0.0
                                                         Injection number                                                                                                                     0.7  0.8  0.9  1.0  1.1  1.2  min  1.00  1.25  1.50  min
                                                                                                                                                                                               Dehydroepiandrosterone  5α-Dihydrotestosterone
                                    Results from Consecutive Analyses of Alprazolam-Spiked Human Blood Plasma                                                                                                     LCMS-8060NX    LCMS-8060
                                                                                                                                                                                                                       LCMS-8060NX
      6                                                                                                                                                                                                                Liquid Chromatograph Mass Spectrometer  7
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