Page 15 - Shimadzu LCMS-8060

Engineered for Robustness and

Easy Operation/Maintenance
Newly Designed Ionization Unit

The robustness of the LCMS-8060 and modified ion optics was a deuterated internal standard (alprazolam-d5) the RSD was 3.5%. Designed without cables or tubes, removing the new ionization unit is simple: release a one-touch lever to open the unit and lift it out. In
also assessed by injecting 2400 samples of femto-gram levels of As part of the robustness test the vacuum system was vented to addition, no tools are needed to detach the needles fitted in APCI and DUIS units, allowing for easy maintenance.
™
alprazolam spiked into protein-precipitated human plasma extracts model a transient power failure with no effect on signal response
over a 6 day period (over 400 samples were injected each day). or baseline noise level.
The RSD of peak area response was 5% over this test period, using

×10 4
7.0 1.4
100 pg/mL alprazolam in human plasma with internal standard
m/z 309.05 > 281.10
6.0 1.2
1st day 2nd day 3rd day 4th day 5th day 6th day
5.0 A week 1.0
Peak Area 4.0 Area Ratio 3.46 %RSD 0.8 Area Ratio
Area Ratio 3.46 %RSD
0.6
3.0
2.0 0.4
Peak Area 4.97 %RSD
1.0 0.2
Vacuum
Pause in batch Pause in batch system Pause in batch Pause in batch
analysis analysis vented analysis analysis
0.0 0.0
0 400 800 1200 1600 2000 2400
Injection number
Long term stability study on LCMS-8060
(×10,000) (×10,000) (×10,000)
1.00 1.00 1.00
1 st injection 1200 th injection 2400 th injection
0.75 0.75 0.75
0.50 0.50 0.50
0.25 0.25 0.25
0.00 0.00 0.00
0.8 0.9 1.0 1.1 1.2 1.3 min 0.8 0.9 1.0 1.1 1.2 1.3 min 0.8 0.9 1.0 1.1 1.2 1.3 min
MRM chromatograms for the 1st, 1200th and 2400th measurements of alprazolam
Steps for ESI Capillary Replacement
Intraday and Interday variations on LCMS-8060
Intraday Variation (%RSD) Interday Variation (%RSD)
Compound
1st day 2nd day 3rd day 4th day 5th day 6th day Days 1–3 Days 4–6 6 Day Total 3
1
Alprazolam 5.04 4.94 5.06 5.38 4.55 4.83 3.19 1.63 2.74
2
Alprazolam-d5 (ISTD) 5.04 4.68 5.48 5.31 4.26 4.91 2.62 1.89 2.18
ESI Capillary
Area ratio
(Alprazolam / Alprazolam-d5) 3.48 3.11 3.48 3.44 3.71 3.54 1.79 0.26 1.40

Easy System Maintenance Reduces Downtime ESI (standard) APCI (optional) DUIS (optional)

As with Shimadzu’s other triple quad systems, maintaining the LCMS-8060 is simple. Replacing the desolvation line (DL) and ESI capillary
is quick and easy. Additionally, the design allows users to replace the DL without breaking vacuum, providing greater uptime and usability.

Steps for DL Replacement

2
1

3
DL (Desolvation Line)

14 Liquid Chromatograph Mass Spectrometer LCMS-8060 15