Page 7 - Shimadzu LCMS-8040

Higher Sensitivity for MS/MS Acquisition Modes

The increased scan sensitivity of the LCMS-8040 also applies to Mass error 300.0
scan modes specific to triple quadrupole mass spectrometers, such associated with a
rise in scan speed
as product ion scanning, precursor ion scanning, and neutral loss
scanning. Historically, mass spectrometers have introduced mass
deviation in linked scans, such as precursor ion scans or neutral loss 300.5
scans, when measured at maximum scan speeds.

Precursor ion of m/z 149 Dipropyl phthalate Increase in Scan Speed
Inten.(×10 5 ) Inten.(×10 5 ) 301.0
251.25 7.5 251.25
CH3
5.0
5.0
2.5 CH3 298.0 299.0 300.0 301.0 302.0 303.0 m/z
2.5
m/z 149
Typical triple quadrupole mass spectrometer
0.0 0.0
240.0 250.0 m/z 240.0 250.0 m/z
Inten.(×10 7 ) Inten.(×10 7 )
2.0
2727 u/sec 6000 u/sec
8040 However, Shimadzu's proprietary UFscanning technology allows
1.0 1.5 performing precursor ion scans or neutral loss scans at high speeds
8030
1.0 without loss of mass accuracy. In addition, the LCMS-8040 offers
0.5 higher sensitivity levels. Precursor scan results for eight kinds of
0.5 phthalate esters are shown to the left. Scans were performed at
two speeds, 2727 u/sec and 6000 u/sec. No mass shift is observed
0 0
3.0 4.0 5.0 min 3.0 4.0 5.0 min at either scan speed, and a significant sensitivity improvement is
Precursor Ion Scan of Standard Mixture Sample of 8 Phthalate Esters observed for the LCMS-8040.
Easy Method Transfer
MRM parameters for the LCMS-8030 can be used in LCMS-8040 systems.
Optimization of MRM parameters is an early step in LCMS method employing LCMS-8030 MRM conditions will be able to transfer
development. Shimadzu has streamlined the MRM optimization these MRMs directly to the LCMS-8040. To demonstrate this, a
process with a rapid and simplified approach based on automated method for the simultaneous analysis of 167 pesticides was
flow injection analysis. Several method packages, which contain transferred without modification from the LCMS-8030 to the
chromatographic and optimized MRM conditions for a variety of LCMS-8040. Increased sensitivity was obtained for all compounds;
analytes, including residual pesticides, veterinary drugs, and three example chromatograms are shown below.
forensic drugs of abuse, have also been released. Laboratories

10 ppb: Cloquintocet-mexyl(9.5X) 10 ppb: Pyrazosulfuron-ethyl(7.4X) 10 ppb: Linuron(5.2X)
(×100,000) (×100) (×1,000)
1.50 ESI + 336.20>238.00 ESI + 415.20 >182.10 ESI 246.90 >160.00
1.25
1.25 8040 8040 1.50 8040
1.00
1.00
0.75 1.00
0.75
0.50
0.50
0.50
0.25 8030
0.25 8030
8030
0.00 0.00
0.00
18.0 19.0 min 5.5 6.0 6.5 7.0 min 14.0 14.5 15.0 min

L i quid C h r o mat o g r aph Mas s Sp e c tr omet er 7