Page 5 - Shimadzu LCMS-8040

Higher CID Efficiency with Improved Collision Cell

UFsweeper II

The UFsweeper II is a high-sensitivity, high-speed collision cell that
features improved ion focusing by using high-speed ion transport
technology. This yields better product ion transmission in the
collision cell, maintaining signal intensity and suppressing crosstalk,
even for high-speed or simultaneous multi-component analysis. The
capability for high-throughput analysis is thus maintained at lower
levels of detection.

Precursor Product LCMS-8030 LCMS-8040 Proportional
Sample Name
m/z m/z CID efficiency CID efficiency Increase
Lidocaine 235.4 86.1 20.0 29.0 1.4
Atropine 290.1 124.1 7.2 11.0 1.5
Yohimbine 355.1 144.0 9.5 15.6 1.6
Tetracaine 265.1 176.1 15.7 32.0 2.0
Doxepin 280.1 107.1 4.4 7.2 1.6
Imipramine 281.1 86.1 16.2 22.1 1.3
Nortriptyline 264.1 233.1 3.8 7.4 1.9
Isopropylantipyrine 231.1 188.9 1.5 2.9 1.9
Diazepam 285.0 154.0 2.4 3.9 1.6
Reserpine 609.3 195.1 3.9 6.6 1.7

The LCMS-8040 offers higher sensitivity while maintaining the measured simultaneously, both the LCMS-8030 and LCMS-8040
ultrafast performance of the LCMS-8030. The figures below show accurately identified all 167 components. In addition, the LCMS-8040
simultaneous analysis of 167 pesticides in MRM positive/negative showed improved sensitivity – an average of three times higher for
ion analysis mode. Even though positive and negative ions were all components.

LCMS-8030 LCMS-8040
(×10,000,000) (×10,000,000)
1.00 1.00

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
5.0 10.0 15.0 20.0 min 5.0 10.0 15.0 20.0 min
HPLC MS
Analysis Column Shim-pack FC-ODS (2.0 mmI.D. × 150 mmL., 3 μm) Probe Voltage +4.5 kV (ESI-Positive mode) / −3.5 kV (ESI-Negative mode)
Mobile Phase A 5 mmol/L ammonium acetate - Water Nebulizing Gas Flow 1.5 L/min
Mobile Phase B 5 mmol/L ammonium acetate - Methanol Drying Gas Flow 10 L/min
Gradient Program 15%B (0 min) – 40%B (1–3.5 min) – 50%B (6 min) DL Temperature 250°C
– 55%B (8 min) – 95%B (17.5–30 min) – 15%B (30.01–40 min) Heat Block Temperature 400°C
Flow Rate 0.2 mL/min
Column Temperature 40°C

L i quid C h r o mat o g r aph Mas s Sp e c tr omet er 5