Page 11 - Shimadzu iMScope QT

Matrix Vapor Deposition System
iMLayer (Option)

Applying the matrix by the vapor deposition method supports high resolution MALDI imaging.

Inside iMLayer

Sample plate
Layer thickness
Measurement unit
Body tissue
Sample holder
Sublimation
Shutter
Matrix
Deposition boat
Heating

Applying ne matrix crystals by vapor deposition

Both Spatial Resolution and Sensitivity Thanks
Sample plate
to the Two-Step Vapor Deposition Method
Spraying
Body tissue
• Two-Step Vapor Deposition
Sublimation
A two-step vapor deposition method has been developed*, which
provides high spatial resolution (5 to 10 m) and high sensitivity, Matrix
thanks to a combination of iMLayer (vapor deposition method) and
iMLayer AERO (spray method). This unique experiment can only be Heating
implemented using Shimadzu sample preparation solutions. Vapor Deposition Metod Spray Method
* Patent No.: JP6153139 and JP6183779

Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur
Blur

Hand Spray
• Matrix: 9-AA
• Volume used: 200 µL

Two-Step Vapor Deposition
1 step: iMLayer (vapor deposition method)
• Film thickness: 1 m
2 step: iMLayer AERO (spray method)
• Solution delivery volume: 120 L/min
• Stage speed: 70 mm/sec
• Laminating layers: 4

iMScope QT
Imaging Mass Microscope 11