Page 10 - Shimadzu iMScope QT

Pretreatment Instruments for MALDI Imaging

With mass spectrometry imaging (MSI), suitable pretreatment is important. High quality MSI analysis results are obtained from a
combination of the spray method and the vapor deposition method.

Work Flow

Derivatization/ Matrix spraying MSI analysis MSI
Enzyme treatment data analysis

Automation of pretreatments conventionally requiring expertise

Automatic Sprayer
Sample stage and Spray nozzle
The newly developed spray nozzle provides
iMLayer AERO (Option) a ne spray. The distance between the
sample and nozzle can be adjusted
between 5 and 10 cm.
The iMLayer AERO incorporates a sample stage that moves at a controlled rate while
maintaining the same distance from the spray nozzle, enabling stable matrix spraying.
Over multiple strokes, the sample becomes laminated with ne matrix
crystals, enabling high sensitivity and high spatial resolution.

Rinse solution bottle
Reagent bottle Spray nozzle
Imaging with High Reproducibility

• Humidity Control • Clog-Free Reagent Delivery
Humidity does not impact matrix deposition because the If the matrix clogs the nozzle tip, the spray becomes unstable,
atmosphere within the spray chamber is replaced before which can lead to lower reproducibility. The rinsing mechanism
pretreatment. Spraying can be performed under more stable allows for clog-free stable spraying which enables high
conditions than with a hand spray. reproducibility in MALDI analyses.

iMLayer AERO

Humidity has no impact.

N2 purging Clog Rinse
solution

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