Page 17 - Shimadu Consumables Catalog

Shim-pack Velox HILIC

Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the
retention of challenging polar analytes. Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for this
application.
Orthogonal selectivity to reversed phase chromatography
Increased retention of polar analytes
Increased MS sensitivity
Direct compatibility with sample preparation eluates

Retention of Nucleosides
Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well
retained and separated with Shim-pack Velox HILIC column.

1. Toluene 2. Uracil 3. Uridine 4. Adenosine 5. Guanosine 6. Cytidine

LC Conditions
Column : Shim-pack Velox HILIC, 2.7 µm, 3.0×100 mm
(PN: 227-32026-02)
Column Temp. : 30 ºC
Flow Rate : 0.4 mL/min
Mobile Phase : Acetonitrile/20mM AcONH4aq.=9/1
Detection : UV 254 nm
Sample : 1. Toluene, 2. Uracil, 3. Uridine, 4. Adenosine,
5. Guanosine, 6. Cytidine
Injection Volume : 1 µL

Shim-pack Velox 1.8 µm Columns

Chemistry SP-C18 C18 Biphenyl PFPP
I.D. (mm)
2.1 3.0 2.1 3.0 2.1 3.0 2.1 3.0
Length (mm)
30 227-32001-01 - 227-32007-01 - 227-32013-01 - 227-32019-01 -
50 227-32001-02 227-32002-01 227-32007-02 227-32008-01 227-32013-02 227-32014-01 227-32019-02 227-32020-01
100 227-32001-03 227-32002-02 227-32007-03 227-32008-02 227-32013-03 227-32014-02 227-32019-03 227-32020-02
150 227-32001-04 - 227-32007-04 - 227-32013-04 - 227-32019-04 -

Shim-pack Velox 2.7 µm Columns

Chemistry SP-C18 C18 Biphenyl
I.D. (mm)
2.1 3.0 4.6 2.1 3.0 4.6 2.1 3.0 4.6
Length (mm)
30 227-32003-01 227-32004-01 227-32005-01 227-32009-01 227-32010-01 227-32011-01 227-32015-01 227-32016-01 227-32017-01
50 227-32003-02 227-32004-02 227-32005-02 227-32009-02 227-32010-02 227-32011-02 227-32015-02 227-32016-02 227-32017-02
100 227-32003-03 227-32004-03 227-32005-03 227-32009-03 227-32010-03 227-32011-03 227-32015-03 227-32016-03 227-32017-03
150 227-32003-04 227-32004-04 227-32005-04 227-32009-04 227-32010-04 227-32011-04 227-32015-04 227-32016-04 227-32017-04

Chemistry PFPP HILIC
I.D. (mm)
2.1 3.0 4.6 2.1 3.0 4.6
Length (mm)
30 227-32021-01 227-32022-01 227-32023-01 227-32025-01 - -
50 227-32021-02 227-32022-02 227-32023-02 227-32025-02 227-32026-01 227-32027-01
100 227-32021-03 227-32022-03 227-32023-03 227-32025-03 227-32026-02 227-32027-02
150 227-32021-04 227-32022-04 227-32023-04 227-32025-04 227-32026-03 227-32027-03

Pg A-13