Page 14 - Shimadu Consumables Catalog

Core Shell Columns

Shim-pack Velox SP-C18

Designed and intended specifically for use under low pH condition, Shim-pack Velox SP (Sterically
Protected)-C18 offers a well balanced retention profile with a long life time even under harsh, acidic
condition needed for LC/MS(/MS) analysis.
Sterically protected to resist strongly acidic (pH 1-8) mobile phase condition
Well balanced retention profile
Suitable for LC/MS(/MS) analysis

Low pH Stability

Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns
maintain a stable retention profile under strongly acidic mobile phase condition (pH1) .

Conditions
Flow Rate : 0.6 mL/min
Column Temp. : 60 °C
Mobile Phase A : 0.1 % TFA in water
Mobile Phase B : Acetonitrile
Gradient : 45 % B (0 min – 7.5 min) g 5 % B (7.51 min – 50 min)
100 % B (55 min ) g 45 % B (55.01 min g 60 min)
Shim-pack Velox SP-C18 Brand A FPP C18
Brand B FPP C18
Brand C FPP C18
Brand D Core-shell C18
Brand E Core-shell C18
Brand F Core-shell C18

Simultaneous Analysis of 22 Preservative Agents

More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by
Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide
range of commercial product within acceptable analytical times.

Preservative agents
1. 2-methyl-4-Isothiazolin-3-one 12. Isopropyl Paraben
2. 2-Bromo-2-Nitro-1,3-propanediol 13. Propyl Paraben
3. Salicylic Acid 14. 4-Chloro-3-Methylphenol
4. Isothiazolinones 15. Ethyl Benzoate
5. Benzoic Acid 16. Isobutyl 4-Hydroxybenzoate
6. Benzyl Alcohol 17. Butyl Paraben
7. Sorbic Acid 18. Chloroxylenol
8. 2-Phenoxyethanol 19. Phenyl Benzoate
9. Methyl Paraben 20. Clorofene
10. Ethyl Paraben 21. Triclocarban
11. Methyl Benzoate 22. Triclosan

LC Conditions
Column : Shim-pack Velox SP-C18, 2.7 µm, 3.0×100 mm
(PN: 227-32004-03)
Flow Rate : 1.0 mL/min
Column Temp. : 45 °C
Mobile Phase A : NaH2PO4 aq. (25 mM, pH3.8)
Mobile Phase B : MeOH/ACN = 9/1
Gradient : 8 %B (0 min ) g 30 % B (0.7 min – 2.7 min)
47 % B (2.71 min – 5min ) g 52 % B (6 min)
80 %B (7 min – 8 min) g 8 %B (9.01 min -10 min)
Injection Volume : 1 µL
Conc. : 50 mg/L

Pg A-10