Page 19 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack Scepter LC Columns

Excellent stability & performance could be achieved under a wide range of LC conditions
Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns,
which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter
columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 µm, 3 µm, 5 µm) and different column dimensions, Shim-pack Scepter LC columns are
fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory
instrumentation.
Shim-pack Scepter Chemistries

Reversed Phase HILIC
Shim-pack Scepter
C18 HD-C18 C8 Pheny PFPP C4 Diol-HILIC
Trifunctional C18 Trifunctional C18
Trifunctional
Trifunctional
Ligand Type Generic Purpose High Density Trifunctional C8 Trifunctional Pentafluorophenylpropyl Butyl group Dihydroxypropyl
Phenylbutyl
Type Type
Particle Organic Silica Hybrid
Particle Size 1.9 μm, 3 μm, 5 μm
Pore Size 12 nm 8 nm 12 nm 30 nm 12 nm
End Capping Proprietary None Proprietary None
pH Range 1 - 12 1 - 10 1 - 8 1 - 10 2 - 10
100% aqueous
condition Yes No No Yes Yes No N/A
USP Classification L1 L1 L7 L11 L43 L26 L20

Method Scouting Utilize excellent stability & performance under a wide range of LC conditions

With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting
combining mobile phase pH and organic modifier.
Comparison of Chromatograms using Gradient condition with Acetonitrile

Analytes
1. Saccharin (pKa = 2.2)
2. Dextromethorphan (pKa = 8.3)
3. Amitriptyline (pKa = 9.4)
4. N-Butyl paraben (pKa = 8.3)
5. Ibupurofen (pKa = 4.4)

For Shim-pack Scepter C4, please refer to page A-22

Pg A-15