Page 16 - Shimadu Consumables Catalog

Core Shell Columns

Shim-pack Velox Biphenyl

Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. It is useful for fast separations in
bioanalytical applications due to the increased retention of early eluting analytes such as dipolar, unsaturated and
conjugated analytes.
S i
O
Complementary selectivity to alkyl phases
Enhanced separation of aromatic compounds
Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis

Separating the Structural Isomers
Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two structural
isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in chemical
structures, could be relatively well resolved with shim-pack Velox Biphenyl column.

Amobarbital Pentobarbital
5-ethyl-5-(3-methylbutyl)- 5-ethyl-5-pentan-2-yl-1,3-
1,3-diazinane-2,4,6-trione diazinane-2,4,6-trione
Conditions
System : Nexera UHPLC System / LCMS-8050
Column : Shim-pack Velox Biphenyl 2.7 µm,
2.1 mm × 100 mm (PN: 227-32015-03)
Column Temp. : 30 °C
Flow Rate : 0.5 mL/min
Mobile Phase A : 0.15 mM ammonium fluoride in water
Mobile Phase B : Methanol
Gradient : 20 % B (0 min) g 48 % B (1.5 min) g
53 % B (4 min)
100 %B (6 min - 7.5 min) g 20 % B
(7.51 min - 9.5 min)
Sample preparation : Enzymatically hydrolyzed human urine
was spiked with target compounds
between 10 - 1000 % of the required
cut off concentration. Samples were
subsequently diluted 5x with 0.1 %
formic acid.
Shim-pack Velox PFPP

Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is
suitable for the analysis of halogenated compounds, positional isomers and charged bases.
Alternative selectivity to C18 columns
Suitable for positional isomers and halogenated compounds
Offers increased retention for charged bases

Good Separation of Cis / Trans Stilbene
Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well
separated with Shim-pack Velox PFPP column.

1. cis-Stilbene 2. trans-Stilbene

LC Conditions
Column : Shim-pack Velox PFPP, 2.7 µm, 3.0×100 mm
(PN: 227-32022-04)
Column Temp. : 40 °C
Flow Rate : 0.4 mL/min
Mobile Phase : Methanol/Water = 9/1
Detection : UV 254 nm
Sample : 1. cis-Stilbene, 2. trans-Stilbene
Injection Volume : 1 µL

Pg A-12