Page 13 - Shimadu Consumables Catalog

Column Chemistries

Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best
opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a
wide variety of applications and challenging separations.

SP-C18 C18 Biphenyl PFPP HILIC
USP Classification L1 L1 L11 L43 L3
Sterically protected Pentafluorophenyl
Ligand type C18 Biphenyl None
C18 propyl
Particle Size (µm) 1.8, 2.7, 5 1.8, 2.7, 5 1.8, 2.7, 5 1.8, 2.7, 5 2.7
Pore size (Å) 90 90 90 90 90
2
2
1.8 µm 125 m /g 125 m /g 125 m /g 125 m /g -
2
2
Suraface Area 2.7 µm 130 m /g 130 m /g 130 m /g 130 m /g 130 m /g
2
2
2
2
2
2
2
2
2
5 µm 100 m /g 100 m /g 100 m /g 100 m /g -
1.8 µm 7 % 9 % 7 % 4 % -
Carbon Load 2.7 µm 7 % 7 % 7 % 4 % NA
5 µm 5 % 5 % 5 % 3 % -
End-Cap No YES YES No No
pH range 1.0-8.0 2.0-8.0 1.5-8.0 2.0-8.0 2.0-8.0
1.8 µm 100 MPa* 100 MPa* 100 MPa* 100 MPa* -
Max Pressure 2.7 µm 60 MPa 60 MPa 60 MPa 60 MPa 60 MPa
5 µm 40 MPa 40 MPa 40 MPa 40 MPa -
* For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 80MPa.
Lot to Lot Reproducibility
We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance. From one
lot to the next, every Shim-pack Velox column you purchase will perform consistently.

Compounds (50 ng/mL)
1. Cortisol
2. 11-Deoxycortisol
3. Estradiol
4. Boldenone
5. Testosterone
6. Androstenedione
7. Progesterone
Conditions
Column : Shim-pack Velox Biphenyl 2.7 µm, 3.0×100 mm
(P/N: 227-32016-03 )
Flow Rate : 0.7 mL/min
Column Temp. : 30 °C
Sample Dilulent : Initial mobile phase
Injection Volume : 5 µL
Mobile Phase A : 0.1 % formic acid in water
Mobile Phase B : 0.1 % formic acid in acetonitrile Gradient: 40 % B (0 min)
80 % B (3 min)

Pg A-9