Page 3 - MUP-3100

Performs dispensing, the addition of reagents, heating,
centrifugation, solid phase column extraction, and sample
Fully Automated Sample Preparation Module for Glycan Analysis delivery, automating the sequence of glycan pretreatment *
Eliminates human errors and variations by automation
Prevents mistakes by checking the positioning of the reagents
via image processing before beginning automated operations,
Automatic Pretreatment of Modified Glycans from supporting a stable workflow
* The reagents included in the kit are prepared in advance by the analyst.
Antibodies in Culture Supernatants

Stable performance eliminates differences between analysts,
providing peace of mind
Simply set the reagents prepared with samples and kits into the
device to automatically perform pre-processing and complete it
The structure of glycans in antibody drugs may affect the stability, function, and structural integrity of without manual intervention
biopharmaceuticals; as a result, glycan profiles are evaluated for quality control purposes.
Stores the automatically generated preparation results report in
Glycan structures vary considerably depending on the cultivation conditions, and non-uniformity cannot be avoided, so it the database
is controlled even in the production process.
Equipped with error log and movie saving function in case of
trouble and to ensure traceability
HPLC and LC/MS are used for the profiling and structural analysis of modified glycans from antibodies in the culture
supernatants of antibody-producing cells. The pretreatment sequence, including the purification of antibodies from
culture supernatants, glycan cut-out, and fluorescent labeling, is complicated and typically requires at least two days.

Shimadzu developed the MUP-3100 Fully Automated Sample Preparation Module for Glycan Analysis in conjunction with
Sumitomo Bakelite Co., Ltd., for use with their antibody N-glycan analysis kit.
Through the combination of MUP-3100 and the kit, complicated
The MUP-3100 eliminates manual sample preparation, increases overall analysis throughput, and improves reproducibility. procedures can be automatically processed in 6 hours
Accommodates N-linked glycans (N-glycan) in antibodies,
allowing simultaneous processing of up to 24 samples
Possible to verify the profiling of glycosylation from antibodies
in culture supernatant, towards achieving an efficient workflow
for optimizing culture conditions

Auto-EZGlyco mAb-N Kit for SHIMADZU
™
(Sumitomo Bakelite Co., Ltd.)

This is capable of everything from purifying antibodies from
unpurified samples (e.g. cell culture solution) and other partially
purified samples, to glycan cut-out, fluorescent labeling of glycans and
glycan purification. The kit also includes necessary reagents such as a
ta glycan release enzyme and a glycan fluorescent labeling reagent.