Page 33 - Application Notebook - Solution for Food Development

C190-E189A

Comprehensive Two-Dimensional Liquid Chromatograph
Nexera-e

Catch the peaks behind the peaks

The Nexera-e comprehensive two-dimensional liquid chromatograph (LC×LC)
effectively separates samples with complex constituents, including pharmaceutical
impurities, proteolytic enzyme digests, natural materials such as food extracts, and
synthetic polymers.
The dedicated LC×LC-Assist and ChromSquare software provide excellent usability and
visibility, and allow the analyst to quickly analyze a large number of sample components.

Achieving High-Resolution Analysis with LC×LC

Comprehensive two-dimensional liquid chromatography (2D-LC) is a completely new analytical technique combining two different separation modes
orthogonally to provide a substantial improvement in separation performance. Combining multiple separation modes enables the separation of
components that conventional one-dimensional liquid chromatography has difficulty separating, and allows the analysis of complex samples.
Comprehensive 2D-LC also differs from conventional 2D-LC by acquiring data that show the results of both first- and second-dimension separations,
which allows the analyst to maximize the effectiveness of both separating systems.
Time programming for the second-dimension separation is configured in detail
based on target component elution times and eluate solvent concentrations from
the first-dimension separation, which allows the discovery of components otherwise
hidden among target components.
LC×LC-Assist and ChromSquare are dedicated software for the Nexera-e. They offer
excellent operability and visibility, and provide powerful support for LC×LC analysis.

Blue: First-dimension time program
Red: Second-dimension time program

Example Analysis of Glycyrrhizic Acid in Kakkonto