Page 32 - Application Notebook - Solution for Food Development

Application No.L491
News

n Quantitation of β-Carotene in Red Chili Pepper separation obtained with the Nexera-e.
by LC/MS/MS Fig. 3 shows the two-dimensional separation data of
The analytical conditions are shown in Table 1, and the β-carotene obtained from DUIS-positive mode MRM
sample pretreatment conditions are shown in Fig. 2. analysis of the calibration curve, and Fig. 4 shows the
β-carotene, which is a precursor of vitamin A, was linearity of the three values (blobs) in the range of 0.01
detected in the two-dimensional separation of the to 10 mg/L, which correspond to the peak volumes
carotenoids. Quantitation was then conducted using used for quantitation. The correlation coefficient (r) =
the LCMS-8030 triple quadrupole mass spectrometer. 0.998976 indicates results with good linearity. The
Both high sensitivity and high selectivity can be quantitative result for β-carotene present in red chili
obtained using MRM analysis, and further, reduced ion pepper was calculated as 1.22 mg/L based on the
suppression can be expected with the two-dimensional concentration in the final sample following extraction.

Table 1 Analytical Conditions
1D Colum : Ascentis Cyano (250 mm L. × 1.0 mm I.D., 5 µm)
Mobile Phase : A; Hexane
B; Hexane/Butylacetate/Acetone = 80/15/5 (v/v/v)
Flowrate : 0.02 mL/min
Time Program : B Conc. 0 % (0.01 min) → 0 % (5 min) → 100 % (65 min) → 100 % (75 min) → 0 % (76 min)
Column Temp. : 30 °C
Injection vol. : 2 µL
Loop vol. : 20 µL
2D Column : Ascentis Express C18 (30 mm L. × 4.6 mm I.D., 2.7 µm)
Mobile Phase : A; acetnitrile
B; 2-propanol
Flowrate : 4 mL/min (0.8 mL/min split for MS)
Time Program : B Conc. 0 % (0.01 min) → 50 % (0.17 - 0.54 min) → 80 % (0.54 - 0.93 min) → 30 % (0.94 min) → STOP (1 min)
Detector : SPD-M30A Photo diode array detector (standard cell, wave length = 450 nm)
Shimadzu LCMS-8030 (DUIS positive mode, targeted β-carotene MRM transition: m/z 536.40 > 444.30)

)PNPHFOBUFE SFE DIJMMJ QFQQFS
Capsicum annuum L. H

"EE DPOTFDVUJWF N- BMJRVPUT PG NFUIBOPM
FUIZM BDFUBUF QFUSPMFVN FUIFS W W W
Area = 551615
$PNCJOF FYUSBDUT BOE pMUSBUF Concentration = 1.22 mg/L

&WBQPSBUF UP ESZOFTT VOEFS WBDVVN $

Fig. 3 2D Plot of β-Carotene
%JTTPMWFE JO N- NFUIBOPM
UFSU CVUZM NFUIZM FUIFS W W

'JMUSBUF BOE JOKFDU µ- JOUP )1-$

Correlation
Fig. 2 Sample Preparation Coefficient (r) = 0.998976

Conc. (mg/L)

Fig. 4 Linearity of Calibration Curve for β-Carotene

Data provided by University of Messina Prof. Luigi Mondello and Chromaleont S.r.l.

First Edition: May. 2015

For Research Use Only. Not for use in diagnostic procedures.
The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu.
The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its
accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the
use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject
to change without notice.
www.shimadzu.com/an/ © Shimadzu Corporation, 2015