Page 42 - Solutions for Plastic Evaluation

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Product Information
MALDI-TOFMS
Evaluation of Plastic Materials

Evaluation of Plastic Materials Analysis of Trace Components in Synthetic Polymers Using an SEC-AccuSpot-AXIMA System
SEC-MALDI-TOFMS systems provide a useful method of analyzing trace components in synthetic polymer samples by first using
size exclusion chromatography (SEC) to fractionate samples by size and then using MALDI-TOFMS to measure molecular weight
information from each fraction. Combining the organic solvent resistant Accuspot system with the AXIMA MALDI TOFMS
integrates the entire process from separation by SEC to analysis by MALDI-TOFMS. Therefore, SEC-AccuSpot-AXIMA systems
provide a powerful tool for analyzing trace components.

SEC-AccuSpot-AXIMA System
Evaluation of Raw Materials mixed together, the components can mutually inhibit ionization, which results in detection of only the primary components, leaving
MALDI-TOFMS is widely used as a method for characterizing synthetic polymers. However, if multiple kinds of components are
trace components undetected. The SEC-MALDI-TOFMS method can provide a useful way to avoid that problem by first separating
multi-component samples into different fractions. In a typical SEC-MALDI-TOFMS workflow, mixing matrix and cationizing reagents
with a large number of fractions for MALDI measurement followed by spotting the fractions onto a MALDI sample plate, is time-
consuming and impractical. In contrast, using an SEC-AccuSpot system, which mixes matrix or other reagents with fractions from
SEC and successively loads them onto MALDI sample plates, provides a fully integrated process of SEC fractionation and sample
spotting for measurement by MALDI-TOFMS. Using the SEC-AccuSpot-AXIMA system reduces the total analysis time to 1/4 the
time required previously. Therefore, SEC-AccuSpot-AXIMA systems provide a powerful tool for analyzing trace components.
Product Evaluation Prominence Online AccuSpot AXIMA Performance

Product Information SEC (microscale) MALDI plate MALDI-TOFMS

Plate sensor MALD plate

Before spotting Spotting After spotting

SEC-AccuSpot-AXIMA System

Features of the Organic Solvent Resistant AccuSpot System
▪ The AccuSpot is an automatic spotting system that mixes matrix or other reagent
with effluent from the LC unit and then successively spots the mixture onto MALDI
sample plates.
▪ Installing an optional GPC compliance kit enables using organic solvents as a
mobile phase. GPC Compliance Kit
• Flow line parts are changed from PEEK to stainless steel or PTFE-based materials.
• An exhaust fan draws any gases from residual organic solvents out of the
instrument and into the fume hood.
▪ The plate changer function enables continuous spotting of up to 9 MALDI plates.
▪ Computer interface enables central control of spotting parameters.
▪ Spotting monitor functionality is included standard
Before spotting Spotting After spotting
• The standard-equipped CCD camera allows monitoring of the actual spotting AccuSpot
process via the control computer.

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