Page 33 - Oligonucleotide Therapeutics Solution Guide

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TORAST-H Series This is a series of high-end consumables that Shimadzu GLC has
evaluated and verified using the latest technology.

Low Adsorption Tip for Micro Pipette/Plate Quantification of samples and reagents before analysis: Modification

TORAST-H Tip/TORAST-H 96well 500RU Analytical Balances Target selection

AP W-AD Series with Automatic Door

• Fast response and high stability
• Superior Quality Control
benefits • Smart Auto Door Improves Measurement Workability
benefits • LabSolutions Balance for Data Reliability Excision

Adsorption Test Using Trypsin Digestion Products of Myoglobin (approx. 8.5 pmol/µL) The standard movable windshield inner plate Auto Door with Touchless Sensor and Unprotected Oligomer synthesis
and ionizer improve stability and responsiveness. Automatic Learning Function
Since most of the micropipette tips used for sample preparation are made of PP, it is necessary to suppress the hydrophobic adsorption to the tip at
low concentration samples. To address this issue, Shimadzu GLC has developed a low adsorption capacity (TORAST-H Tip 200) tip that chemically The smaller the volume in the weighing chamber, the less the effect of Auto door with touchless sensor improves user's work efficiency. This is
bonds a nonionic hydrophilic group to the surface of the 200 µL PP tip. convection and air flow. The model with a minimum display of 0.01 because it is touchless and allows non-contact weighing work without
mg is equipped with a movable windshield internal plate as standard. touching the operation keys, allowing the user to work while holding
By moving up and down according to various containers and samples, the spatula in his hand. In addition, the AP series door with automatic
anyone can carry out stable weighing work. In addition, this equipment learning function allows you to freely set the opening and closing
is equipped with a windless type ionizer STABLO-AP that can eliminate range of all doors to minimize the effects of outside air. The multi-
uV
300000 the effects of static electricity in the weighing chamber as standard function mode allows you to assign a total of four individual functions
290000 12 Peak Peak Peak Peak Peak Peak Peak
# # # # # # # Purification
280000 10 11 12 13 14 15 16 equipment. Ionizer quickly removes static electricity, improving depending on how long you hold your hand over the sensors. The
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TORAST-H ™ Tip 100% 100% 100% 100% 100% 100% 100%
260000 reproducibility and work efficiency. main unit need not be touched, making it ideal for working with toxic
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Vendor A 8.5% 35.7% 28.7% 16.5% N.D. N.D. N.D. substances, and gloves can be worn without interfering with accurate
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Vendor B 5.9% 34.8% 33.3% 33.3% N.D. N.D. N.D. operation.
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200000 Torast-H ™ Tip
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Vendor A Polypropylene tip 200 µL 15
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13
170000 Vendor B Polypropylene tip 200 µL 14
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10
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13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 18.0 min
Highly hydrophobic peptides with a retention time of approximately 14 ~ 18 minutes were adsorbed onto commercially available PP tips, but significant suppression of adsorption was confirmed with the Quality Control
TORAST-H Tip. Characteristic analysis
Realization of High Reproducibility by using a Low-adsorption Well Plate
The low adsorption 96 well plate has a non-ionic hydrophilic group fixed on the inside surface of the well.
Complies with ER / ES Related Regulations
One of the most hydrophobic basic compounds is imipramine. Here, the adsorption result of imipramine to the 96 well plate by LC/MS was shown.
The TORAST-H 96 well 500 RU showed a remarkable adsorption suppression effect compared with the non-coated 96 well plate and the low- Many analytical instruments can prevent data tampering and store analysis
adsorption 96 well plate of other companies. The TORAST-H 96 well 500 RU showed high reproducibility as a result of evaluating the dispersion of conditions and operation history in order to ensure the reliability of the data. >>Balance Technical Information Movies DDS
adsorption compared with the non-coated well plate. LabSolutions Balance is a breakthrough software that solves this challenge for
Intensity electronic balances.
(x1,000) Imipramine 281.35(+) Pharmacokinetics
3.6 TORAST-H ™ 96well 500 RU （Area%: 100）
3.5 Vendor A low adsorption 96well plate Area%: 58.1） AP W-AD series Specification Overview
3.4 Non-coating 96well plate （Area%: 46.7）
3.3 Model AP225W-AD AP135W-AD AP225WD-AD AP125WD-AD AP324W-AD AP224W-AD
3.2 P/N 321-76000-13 321-76000-10 321-76000-12 321-76000-11 321-76000-03 321-76000-02
3.1 Capacity 220 g 135 g 220 g / 102 g 120 g / 52 g 320 g 220 g
3.0 Minimum display 0.01 mg 0.1 mg / 0.01 mg 0.1 mg
2.9 Minimum Weight *1 20 mg *2 200 mg
2.8
2.7 *1 Be compliant with USP Chapter 41. This is the tested value by the weight of the balance’s capacity of 5 % (or 5 grams’ weight). Other
2.6 %RSD The minimum weight value is affected by the installation environment, so it is necessary to measure it in the actual environment of use.
2.5 TORAST-H 96well 500 RU CV:5.3% *2 Measurement conditions of W-AD series (0.01 mg models only) are as follows.
- Set the adjustable windbreak plate in the lowest position
1.75 2.00 2.25 2.50 2.75 3.00 3.25 min Non-coated 96well plate CV:18.7% - With a shield plate configured around the pan
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