Page 15 - Microorganism Species Analysis

MALDI-TOF MS MALDI-TOF MS
Direct Measurements of Microorganisms
Microorganism Solutions Microorganism Solutions

Direct Measurements of Microorganisms Using the MALDI-TOF MS AXIMA Series Data
The conventional analysis of a microorganism-derived component required the extraction, purification, and analysis of the
microorganism (or culture fluid) from which the component is derived. All conventional methods were complex and Direct Measurement of Microorganisms Using the MALDI-TOF MS AXIMA Series
time-consuming. Microorganism Species Observation of
In contrast, MALDI-TOF MS can analyze a wider range of compounds and their states. Test and research flow
This feature allows high throughput and low running costs for analysis of microorganism-derived components for the 1. Pick up some of the bacteria from a single colony pre-cultivated on the plate.
measurement of entire microorganisms. 2. Directly apply the bacteria sample and matrix reagent to the AXIMA MALDI sample plate.
Observation of
A new method using MALDI-TOF MS (Intact-Cell MALDI-TOF Mass Spectrometry) is attracting attention for the 3. Perform mass spectrometry using the MALDI-TOF MS AXIMA Series.
Microorganism Species
measurement of entire microorganisms without complex pretreatment.

Features of Direct Measurement of Microorganisms Using the
MALDI-TOF MS AXIMA Series
MS analysis possible by simply mixing the microorganism with the matrix solution; no complex sample pretreatment Microorganism Species Identification of
required
Analysis complete in just two minutes after the start of measurements
Permits high-throughput analysis (>1,000 samples/day)
Identification of
No pretreatment reagent required – low running costs
Microorganism Species

Sample microorganisms (bacteria) Apply bacteria sample to sample plate
m/z
Fig. 1 MS Spectrum of Escherichia coli (NBRC 3972 Strain) Specific Microorganisms Detection of

Detection of
Fig. 1 shows the direct measurements of Escherichia coli (NBRC 3972 strain). In particular, the ribosomal subunit
proteins (Lxx and Sxx peaks on the spectrum) in the bacteria were detected. In addition, multiple ions derived from
peptides and lipids can be detected directly from the microorganism without extraction or purification.
Specific Microorganisms

Components
Mass spectrometry Components Analysis of Microorganism-Derived

Analysis of Microorganism-Derived

Fig. 2 MS Spectra for Various Bacteria Other Applications
A comparison of the measurement results for various bacteria samples in Fig. 2 shows that distinctive spectral
AXIMA Performance (MS/MS:CID/PSD) AXIMA Confidence (MS/MS:PSD) AXIMA Assurance (MS:Linear) patterns are obtained according to the bacteria species.
Other Applications
Laser Desorption Ionization Time-of-Flight Laser Desorption Ionization Laser Desorption Ionization
Mass Spectrometer Time-of-Flight Mass Spectrometer Time-of-Flight Mass Spectrometer
(Data supplied by Prof. K. Tanamoto and Associate Professor M. Muroi, Faculty of Pharmacy, Musashino University and Mr. Y. Nakagawa,
National Institute of Technology and Evaluation)
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