Page 78 - Application Handbook - Liquid Chromatography

SCA_280_081

High Performance Liquid Chromatography
Analysis of Phenoxyethanol, Sorbic acid and Sodium

dehydroacetate
No. SCA_190_025

Alban Huteau, Shimadzu France SAS.

Introduction Analytical Conditions:
Phenoxyethanol, Sorbic acid, Sodium System configuration:
dehydroacetate are used in many applications
such as cosmetics, food and pharmaceuticals as a A Shimadzu Prominence HPLC system was used
preservative. consisting of a quaternary solvent pumps (LC-
Sorbic acid and its salts, such as sodium sorbate, 20AD) with 3 channel degassers (DGU-20A3R), an
potassium sorbate, and calcium sorbate, are autosampler (SIL-20AC), and a column oven
antimicrobial agents often used as preservatives in (CTO-20AC). The system was also equipped with
food and drinks to prevent the growth of mold, an SPD-M20A photo diode array detector..
yeast, and fungi.
Analytical Method:
Column: Purosper® STAR RP-18e,
125 x 4.0 mm 5 µm with
precolumn
Mobile Phase: A: H2O + 1% Acetic Acid;
B: ACN
Gradient: 20 % B
Flow rate: 1.0 mL/min
Temperature: 35 °C
Injection Volume: 20 µL
Detector: SPD-M20A, semi-micro cell,
sampling rate 40 Hz, time
constant 0.025 sec, slit width 8 nm