Page 77 - Application Handbook - Liquid Chromatography

Application No.L481
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■ Linearity Table 3 Content of Respective Sugars in Energy Drinks
Fig. 3 shows the calibration curves generated using the Content (g/L)
analytical conditions of Table 2. When generating the Energy Drink A Energy Drink B
curves for the six components over a concentration Glucose ND 59
range of 0.2 to 10 g/L (using the average of three area Fructose ND 101
values, respectively), excellent linearity with a coefficient Xylitol 25 ND
2
of determination greater than R =0.9999 was obtained Sorbitol 14 ND
for each component.

Area (×10 ) 4 Area (×10 ) 4
150 150
m e s o t l a e s oc u l g uRI
10
100 100 ■ Peaks
5. xylitol
6. sorbitol
50 50 8 5

R² = 0.9999981 R² = 0.9999992
0 0 6
0 5 10 0 5 10
Concentration (mg/L) Concentration (mg/L)
Area (×10 ) 4 Area (×10 ) 4
150 150 4 6
fructose mannitol
100 100
2
50 50
0
R² = 0.9999976 R² = 0.9999975
0 0
0 5 10 0 5 10
Concentration (mg/L) Concentration (mg/L) 0 10 20 30 40 min
Area (×10 ) 4 Area (×10 ) 4
150 150
xylitol sorbitol
Fig. 4 Chromatogram of Energy Drink A (10 µL Injected)
100 100

50 50

R² = 0.9999982 R² = 0.9999967
0 0 uRI
0 5 10 0 5 10 ■ Peaks
Concentration (mg/L) Concentration (mg/L) 2. glucose
30
3. fructose
Fig. 3 Calibration Curves of a Standard Mixture of Six Sugars
(0.2 – 10 g/L, 10 µL Injected) 3
20
2
■ Analysis of Energy Drink
Figs. 4 and 5 show the chromatograms obtained from
measurement of energy drinks A and B, respectively. 10
Energy drink A was diluted 10:1 with water, and energy
B, 20:1 with water, and after each was filtered through
a 0.2 µm membrane filter, 10 µL of each sample was
injected. The analytical conditions were the same as 0
those of Table 2.
Xylitol and sorbitol were detected in energy drink A, 0 10 20 30 40 min
and glucose and fructose were detected in energy drink
B. Table 3 shows the quantities of each of these sugars
in the respective energy drinks. Fig. 5 Chromatogram of Energy Drink B (10 µL Injected)

First Edition: Jan. 2015

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