Page 77 - Application Handbook - Liquid Chromatography
P. 77

Application  No.L481
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            ■ Linearity                                            Table 3  Content of Respective Sugars in Energy Drinks
            Fig. 3 shows the calibration curves generated using the                      Content (g/L)
            analytical conditions of Table 2. When generating the                Energy Drink A  Energy Drink B
            curves for the six components over a concentration      Glucose          ND             59
            range of 0.2 to 10 g/L (using the average of three area   Fructose       ND             101
            values, respectively), excellent linearity with a coefficient   Xylitol  25             ND
                                       2
            of determination greater than R =0.9999 was obtained     Sorbitol        14             ND
            for each component.


              Area (×10 ) 4         Area (×10 ) 4
              150                   150
                  m   e s o t l a           e s oc u l g          uRI
                                                                  10
              100                   100                                                        ■ Peaks
                                                                                                 5. xylitol
                                                                                                 6. sorbitol
               50                   50                             8                     5

                       R² = 0.9999981        R² = 0.9999992
               0                     0                             6
                 0     5      10      0      5     10
                     Concentration (mg/L)  Concentration (mg/L)
              Area (×10 ) 4         Area (×10 ) 4
              150                   150                            4                       6
                  fructose              mannitol
              100                   100
                                                                   2
               50                   50
                                                                   0
                       R² = 0.9999976        R² = 0.9999975
               0                     0
                 0     5      10      0      5      10
                     Concentration (mg/L)  Concentration (mg/L)     0       10      20      30      40  min
              Area (×10 ) 4         Area (×10 ) 4
              150                   150
                  xylitol               sorbitol
                                                                  Fig. 4  Chromatogram of Energy Drink A (10 µL Injected)
              100                   100

               50                   50

                       R² = 0.9999982        R² = 0.9999967
               0                     0                            uRI
                 0     5      10      0      5     10                                         ■ Peaks
                     Concentration (mg/L)  Concentration (mg/L)                                 2. glucose
                                                                  30
                                                                                                3. fructose
             Fig. 3  Calibration Curves of a Standard Mixture of Six Sugars
                  (0.2 – 10 g/L, 10 µL Injected)                                3
                                                                  20
                                                                             2
            ■ Analysis of Energy Drink
            Figs. 4 and 5 show the chromatograms obtained from
            measurement of energy drinks A and B, respectively.   10
            Energy drink A was diluted 10:1 with water, and energy
            B, 20:1 with water, and after each was filtered through
            a 0.2 µm membrane filter, 10 µL of each sample was
            injected. The analytical conditions were the same as   0
            those of Table 2.
            Xylitol and sorbitol were detected in energy drink A,   0       10      20      30      40  min
            and glucose and fructose were detected in energy drink
            B. Table 3 shows the quantities of each of these sugars
            in the respective energy drinks.                      Fig. 5  Chromatogram of Energy Drink B (10 µL Injected)





                                                                                                      First Edition: Jan. 2015


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