Page 75 - Application Handbook - Liquid Chromatography

Application No.L484
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n Calibration Curve Linearity
8IFBU
Fig. 3 shows the calibration curves generated from
analyses using the conditions of Table 1. Excellent (SJOE MFTT UIBO ŸN
linearity with a coefficient of determination greater
2
than R =0.9999 was obtained for both substances over H
a concentration range of 0.1 to 4 ppm. N- XBUFS BDFUPOJUSJMF W W
4IBLF GPS NJO
"SFB
7BDVVNFE pMUSBUJPO CZ 8IBUNBO (' #
7BDVVNFE pMUSBUJPO CZ 8IBUNBO (' #
7BDVVNFE pMUSBUJPO CZ 8IBUNBO (' #
B /JWBMFOPM

3 N-
N-
$MFBO VQ CZ NVMUJ GVODUJPOBM DPMVNO
$MFBO VQ CZ NVMUJ GVODUJPOBM DPMVNO
l.VMUJ4FQ z PS l"VUPQSFQ .' 5z
l.VMUJ4FQ z PS l"VUPQSFQ .' 5z
N-
N-
&WBQPSBUJPO CZ / HBT VOEFS ˆ

N- XBUFS BDFUPOJUSJMF NFUIBOPM
'JMUSBUJPO W W W
$POD QQN
"SFB
4BNQMF TPMVUJPO
C %FPYZOJWBMFOPM

3
Fig. 4 Pretreatment

$POD QQN
Fig. 3 Linearity of Calibration Curves for a) Nivalenol and
b) Deoxynivalenol
n Analysis of Wheat
Fig. 4 shows the pretreatment procedure used for The results are shown in Fig. 5 and Fig. 6, respectively.
analysis of wheat. Purification of two varieties of wheat Here, the pretreated sample solution was spiked with
was conducted using two types of multi-function nivalenol and deoxynivalenol to achieve respective
columns, the "MultiSep #227" (Romer Labs Inc.) and concentrations of 1.0 ppm.
"Autoprep MF-T" (Showa Denko K.K.).

N"6 N"6

˙1FBLT ˙1FBLT
/JWBMFOPM /JWBMFOPM
%FPYZOJWBMFOPM %FPYZOJWBMFOPM

NJO NJO

Fig. 5 Chromatogram of Wheat A (Spiked with 1.0 ppm each) Fig. 6 Chromatogram of Wheat B (Spiked with 1.0 ppm each)
(MultiSep #227) (Autoprep MF-T)

First Edition: Feb. 2015

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