Page 20 - Food&Beverages Solutions-for-EtO-Analysis-SSI

2.2 Chilli Powder Analysis - Direct Sample Method

Application Databook

EtO & 2-CE Analysis In Chilli Powder (Direct Sample In Headspace Vial)

¿ As such chilli powder sample preparation into headspace vial for trace level quantitation of EtO
& 2-CE. In this approach, chilli powder sample was directly taken into headspace vial and
analyzed for its EtO & 2-CE content.

¿ Shimadzu have successfully part validated this method by using Shimadzu’s GCMS-TQ8040 NX
with HS-20 NX headspace sampler

APPROACH MODE OF ANALYSIS INJECTOR INSTRUMENT TECHNIQUE

As such sample in headspace vial Dynamic Headspace SPL HS-20 NX - GCMS-TQ8040 NX
(EtO & 2-CE)

System Description

GCMS System GCMS-TQ8040 NX
Headspace Sampler HS-20 NX (Dynamic Headspace)
Capillary Column SH-RTX-VMS (60 m X 0.25 mm ID x 1.4 um df)
Ionization Mode Electron Ionization (EI)
Sample Information EtO & 2-CE in chilli powder
Sample Preparation 100 % (As such sample in HS vial) + 1 uL of Acetonitrile (Diluent)
Spiked Sample Preparation 100 mg chilli powder + 1 uL of 1 ppm standard
100 mg chilli powder + 1 uL of 2 ppm standard
100 mg chilli powder + 1 uL of 3 ppm standard
100 mg chilli powder + 1 uL of 4 ppm standard
100 mg chilli powder + 1 uL of 5 ppm standard

Benefits
Ÿ As such sample preparation in headspace vial (without solvent extraction / without any conversion
or derivatization process)
Ÿ Very less sample preparation time
Ÿ Highly cost effective
Ÿ Shimadzu’s optimized solution of dynamic headspace sampler for the analysis of EtO & 2-CE
outperforming the current regulatory limits

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