Page 16 - Food&Beverages Solutions-for-EtO-Analysis-SSI

1.3 Sesame Seeds Analysis - Conversion Method

Application Databook

Representative Data Results :
Conversion Method – EtO =>2-CE (Liquid & Dynamic) Summary of results is shown in Table 1
2-CE
Area (x1,000) (x100) Liquid Dynamic HS
2.00 80.00>31.00
r 2 = 0.99919 3.0 80.00>44.00 LOQ – 10 ppb Method =>
2500 Overlay – 2 to 20 ppb
1.75 82.00>31.00 (w.r.t sample)
2.5 2-CE 2-CE
1.50
2000
1.25 2-CE 2-CE 2.0 2-CE Linearity levels (On column) 2,5,10,15 & 20 ppb 2,5,10,15 & 20 ppb
1500 1.00 2- C E 1.5
0.75 Linearity levels (w.r.t sample) 10,25,50,75 &100 ppb 10,25,50,75 &100 ppb
1000 2 -CE 1.0
2-CE 2
0.50
500 0.25 2-CE 0.5 r (n=3) 0.99919 0.99835
0.00 0.0
0
0.0 5.0 10.0 15.0 Conc. 8.50 8.75 9.00 9.25
8.50 8.75 9.00 9.25
Figure 1: Calibration curve, overlay of linearity standards & chromatogram
of LOQ solution for 2-CE (Liquid) LOQ level conc. (On column) 2 ppb 2 ppb
2-CE LOQ level conc. (w.r.t sample) 10 ppb 10 ppb
(x1,000)
Area (x100)
4000 80.00>31.00
r2 = 0.99835 Ovelay - 2 to 20 ppb 7.5 80.00>44.00 LOQ - 10 ppb % RSD (n=6) 7.6 3.8
3500 2.5 82.00>31.00 (w.r.t sample)
3000 2.0 S/N 16 44
2500 2-CE 2-CE 5.0 2 -CE
1.5
2-CE
2000 2-CE 2 -CE 2-CE
1.0
1500 2-CE 2 -CE 2 -CE 2.5
1000 0.5 2-CE Spiked LOQ level (On column) 2 ppb 2 ppb
500
0.0 Spiked LOQ level (w.r.t sample) 10 ppb 10 ppb
0.0
0
0.0 5.0 10.0 15.0 Conc. 8.50 8.75 9.00
8.50 8.75 9.00 Avg of % recovery (n=3) 101 % 95 %
Figure 2: Calibration curve, overlay of linearity standards & chromatogram
of LOQ solution for 2-CE (Dynamic)
16