Page 14 - Food&Beverages Solutions-for-EtO-Analysis-SSI

1.2 Sesame Seeds Analysis - Direct Sample Method

Application Databook

Representative Data Results :
Direct Sample Method - Analysis of EtO & 2-CE (Dynamic Headspace) Summary of results is shown in Table 1
EtO
Dynamic Headspace
Area (x1,000) (x100)
7000 8.0 44.00>29.00 Method =>
r 2 = 0.99696 EtO Overlay – 10 LOQ – 10 ppb
1.50 44.00>28.00 EtO 2-CE
to 30 ppb (w.r.t sample)
6000 44.00>14.00
7.0
1.25 Linearity levels (On column) 10,20 & 30 ppb 10,20 & 30 ppb
5000 6.0
E tO Linearity levels (w.r.t sample) 10,20 & 30 ppb 10,20 & 30 ppb
1.00 EtO
4000 5.0
2
r (n=3) 0.99696 0.99503
3000 0.75 4.0
EtO
2000 0.50 3.0
1000 2.0 Level 1 - conc. (On column) 10 ppb
0.25 10 ppb
1.0
0 Level 1 - conc. (w.r.t sample) 10 ppb 10 ppb
0.0 5.0 10.0 15.0 20.0 25.0 Conc. 4.75 5.00 5.25 5.50
4.75 5.00 5.25 5.50
Figure 1: Calibration curve, overlay of linearity standards & chromatogram of LOQ solution % RSD (n=3) 2.2 10.1
S/N 26 28
2-CE
(x100) Level 2 - conc. (On column) 20 ppb 20 ppb
Area (x100)
80.00>31.00
r 2 = 0.99503 2.25 Overlay – 10 80.00>44.00 LOQ – 10 ppb Level 2 - conc. (w.r.t sample) 20 ppb 20 ppb
200 to 30 ppb 82.00>31.00 (w.r.t sample)
1.00
2.00 % RSD (n=3) 2.7 11.7
2-CE
1.75
150
0.75
2 -CE
1.50
1.25 Level 3 - conc. (On column) 30 ppb 30 ppb
100 2 -CE
0.50 Level 3 - conc. (w.r.t sample) 30 ppb 30 ppb
1.00
2 -CE
50 0.75 % RSD (n=3) 9.7 14.1
0.25
0.50
0 0.25 st
0.0 5.0 10.0 15.0 20.0 25.0 Conc. 10.00 10.25 10.50 10.75 Prespike 10 ppb – 1 (Recovery) 97 % 101 %
10.00 10.25 10.50 10.75
Figure 2: Calibration curve, overlay of linearity standards & chromatogram of LOQ solution Prespike 10 ppb – 2 nd (Recovery) 105 % 91 %
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