Page 15 - Food&Beverages Solutions-for-EtO-Analysis-SSI

1.3 Sesame Seeds Analysis - Conversion Method

Application Databook

EtO & 2-CE Analysis in Sesame Seeds (Conversion)

¿ With reference to EURL-SRM Analytical Observation Report, Shimadzu have successfully
optimized & part validated conversion method (EtO=>2-CE) for the trace level quantification of
2-CE in sesame seeds by using Shimadzu GCMS-TQ8040 NX with AOC-20i and AOC-20s (Liquid) /
HS-20 NX (dynamic headspace)

APPROACH MODE OF ANALYSIS INJECTOR INSTRUMENT TECHNIQUE

EtO to 2-CE Conversion Liquid SPL AOC-20i/s- GCMS-TQ8040 NX
EtO to 2-CE Conversion Dynamic Headspace SPL HS-20 NX - GCMS-TQ8040 NX

System Description

GCMS System GCMS-TQ8040 NX
Liquid Sampler AOC-20i and AOC-20s
Headspace Sampler HS-20 NX (Dynamic Headspace)
Capillary Column SH-Stabilwax DA (60 m X 0.25 mm ID x 0.25 um df)
Ionization Mode Electron Ionization (EI)
Sample Information 2-CE in sesame seeds (Conversion method)
Sample Preparation 20 % for Liquid / Dynamic Headspace
Reference Method EURL-SRM Analytical Observation report

Benefits
Ÿ With reference to EURL-SRM report, Shimadzu have optimized conversion method for trace level
quantification of 2-CE.
A) EtO to 2-CE conversion – Analysed by liquid injection
B) EtO to 2-CE conversion – Analysed by dynamic headspace injection
Ÿ Shimadzu’s GCMS-TQ8040 NX with AOC-20i / AOC-20s liquid autosampler & HS-20 NX dynamic
headspace sampler is well compatible for the analysis of EtO & 2-CE

Sample Analysis
Conversion of EtO to 2-CE in sesame seeds and analysed by liquid injection

For accuracy study – Respective standard stock solution of EtO was
prespike and further proceed as mentioned in sample analysis.
Et0 => 2-CE conversion factor (0.55) was applied in calculation
For linearity – Respective level of 2-CE standard solution was post
spiked in matrix blank solution and further calibration curve was
plotted.

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