Page 13 - Food&Beverages Solutions-for-EtO-Analysis-SSI

1.2 Sesame Seeds Analysis - Direct Sample Method

Application Databook

EtO & 2-CE Analysis in Sesame Seeds (Direct Sample In Headspace Vial)

¿ As such sesame seed sample preparation into headspace vial for trace level quantitation of EtO
& 2-CE. In this approach, sesame seeds sample was directly taken into headspace vial and
analyzed for its EtO & 2-CE content.
¿ Shimadzu have successfully part validated this method by using Shimadzu’s GCMS-TQ8040 NX
with HS-20 NX headspace sampler

APPROACH MODE OF ANALYSIS INJECTOR INSTRUMENT TECHNIQUE

As such sample in headspace vial Dynamic Headspace SPL HS-20 NX - GCMS-TQ8040 NX
(EtO & 2-CE)

System Description

GCMS System GCMS-TQ8040 NX
Headspace Sampler HS-20 NX (Dynamic Headspace)
Capillary Column SH-RTX-VMS (60 m X 0.25 mm ID x 1.4 um df)
Ionization Mode Electron Ionization (EI)
Sample Information EtO & 2-CE in sesame seeds
Sample Preparation 100 % (As such sample in HS vial) + 1 uL Acetonitrile (Diluent)
Spiked Sample Preparation 100 mg Sesame Seeds + 1 uL of 1 ppm Standard
100 mg Sesame Seeds + 1 uL of 2 ppm Standard
100 mg Sesame Seeds + 1 uL of 3 ppm Standard

Benefits
Ÿ As such sample preparation in headspace vial (without solvent extraction / without any conversion
or derivatization process)
Ÿ Very less sample preparation time
Ÿ Very cost effective
Ÿ Shimadzu’s optimized solution of dynamic headspace sampler for the analysis of EtO & 2-CE
outperforming the current regulatory limits.

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