Page 7 - Food&Beverage Dioxin in Food

Puri cation of Samples Four Puri cation Steps

GO-xHT

GO-xHT is an easy-to-operate automated system Step1
offering high throughput as well as the additional Select the ø18 column Filter and Column Size ø20 column Filter and Column Size
advantage of using less solvents and consumables. column to use.
It provides labs with high-quality extraction as well AgNO3-Silica gel 1 1 AgNO3-Silica gel 1 1
ø15.6 × 81.3
ø17.6 × 116.9
as high return on investment thanks to an
innovative ow path system. H2SO4-Silica gel 2 2 H2SO4-Silica gel 2 2
ø15.6 × 107
ø17.6 × 100
Carbon 3 3 Carbon 3 3
ø6 × 38 ø6 × 38

Alumina 4 Alumina 4
ø6 × 38 ø6 × 38

■Heating puri cation
・The quickness and selectivity of your current DXNs and PCBs analysis can be improved by using this technique. Point Step 2
1
■Less than 100 mL of solvent per sample Assemble the column and Clap!
・It uses only 90 mL of hexane and 2 mL of toluene, without dichloromethane. Thus, this system is both user and eco friendly. Point apply the sample to it.
2
■No cross contamination in the valve-less system 1 3
Clap!
・There are no solenoid valves on the DXNs and PCBs ow lines. All columns and
tubings are disposable. Thus, there is no possibility of cross contamination. 4
2
*Please consult the relevant laws and regulations in your country before going for Clap!
disposal. Point Less than 100 mL
2
of solvent

The bene t of a heated concentration Step 3 Set up the column to the system.
A unique method for column is that the amount of toluene
solvent ow switching necessary for elution of DXNs and
1 Clean up with 90 mL of Hexane with pinch valves. PCBs elution is decreased.
2 Elute PCBs fraction with 0.9±0.1 mL of Toluene

3 Elute DXNs fraction with 1.3±0.1 mL of Toluene
2

1
Step 4 Start puri cation using the

method les for DXNs and
Point 3 PCBs. Note that the method
1 Heating puri cation
The bene t of the heated puri cation DXNs fraction PCBs fraction les are prepared in advance
column is that DXNs and PCBs can be and do not need to be created.
eluted quickly and a chemical reaction 17 isomers of 2,3,7 & 8 substituted 6 isomers of indicator PCBs, 8
of sample matrices is facilitated with Chlorinated dibenzo-p-dioxins, dibenzofurans isomers of mono-ortho PCBs in
the chemically-modi ed silica gel. and 4 isomers of non-ortho PCBs in 1.3 ±0.1 0.9 ±0.1 mL of Toluene
mL of Toluene

6 7