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Mass Imaging of Subcutaneous Tissue

Data Subcutaneous Tissue Mass Imaging of
The skin consists of epidermis, dermis, and subcutaneous tissue layers and serves
to protect the body from external factors. The skin also serves a sensory function,
Results from mass imaging of subcutaneous tissue of a rat are shown in Fig. 1. Based on their m/z value, respective lipids were with acceptors for our sense of touch, temperature, pain, etc. The skin’s ability to
inferred from scientific literature * and using Lipid Search (from Mitsui Knowledge Industry) and the Lipidomics Gateway retain moisture, one of its key functions, involves sebum secreted from Analysis of
(http://www.lipidmaps.org/). sebaceous glands to coat the skin surface, amino acids and other water-retentive Comprehensive
Ceramides (m/z 662.58 (d18:0/22:0)), which attract attention with their ability to retain water, are only present in the horny layer components in the horny cells, lipids between horny layer cells, and other Glycerophospholipids
under the skin surface, whereas sphingomyelin (m/z 713.41 (d18:1/C14:0)) is distributed throughout the entire subcutaneous tissue. factors. Of these factors, intercellular lipids in the horny layer have been
identified as playing a particularly important role. Ceramides are especially well
* PLOS ONE, November 2012, Volume 7, Issue 11
known as a characteristic component of intercellular lipids in the horny layer.
With the growing interest in beautiful skin, researchers are actively searching for
new lipids that are able to retain moisture. A scientific understanding of the skin
Horny layer
Epidermis is also important in the field of pharmaceuticals, such as for evaluating Technology
transdermal drugs. Supercritical Fluid Lipid Analysis Using
Sebaceous
gland
Dermis
Muscle layer
m/z 646.49 m/z 618.30 m/z 730.63
Optical Microscope m/z 662.58 Ceramide-1-phosphate Ceramide-1-phosphate Ceramide-1-phosphate
image Cer(d18:0/22:0) (d18:1/C18:0) (d18:1/C16:0) (d18:1/C24:0) Analysis of
MALDI-MS imaging Technology Glycerophospholipids

Imaging mass spectrometry allows biological molecules and metabolites
90
to be measured directly with a mass spectrometer, while retaining intensity (%) 100
80
information about their position on the sample (tissue section). 70
Subsequently, an image of the two-dimensional distribution of specific 60
50
ions can be created based on the positional information obtained from 40
measurements and the signal intensity of the specific ions in the mass 30 Blood Serum
20 Analysis of Lipid
spectrum (mass imaging). Imaging mass spectrometry also offers other 10
m/z 713.41 m/z 741.53 m/z 769.55 m/z 796.52 m/z 798.54 Mediators in Human
Sphingomyelin Sphingomyelin Sphingomyelin Phosphatidylcholine Phosphatidylcholine advanced features, such as simultaneous detection of multiple 0 600 620 640 660 680 700720 740760 780800 820840 860880 900
(d18:1/C14:0) (d18:1/C16:0) (d18:1/C18:0) (C16:0/C18:2) (C16:0/C18:1) compounds without labeling.

m/z 844.51 m/z 734.56 m/z 756.53 m/z 870.58 m/z 820.53 of Glycolipids Structural Analysis
Phosphatidylcholine Phosphatidylcholine Phosphatidylcholine Phosphatidylethanolamine Phosphatidylcholine
(C16:0/C22:6) (16:0/16:0) (16:0/16:1) (44:6) (36:4) Content of Human ES Cells Composition in Overall Lipid Analysis of Fatty Acid

Imaging Mass Microscope
Overlay
m/z 824.56 m/z 869.69 m/z 895.71 m/z 897.72 m/z 662.59 iMScope TRIO
Phosphatidylcholine Triacylglycerol Triacylglycerol Triacylglycerol m/z 772.52 Esters by GC Acid Methyl
(36:2) (C16:0/C16:0/C18:2) (C16:0/C18:2/C18:1) (C16:0/C18:1/C18:1) m/z 796.52 Analysis of Fatty
Reference: iMScope TRIO brochure (C146-E267)
Fig. 1 Mass Images of Subcutaneous Tissue of a Rat iMScope TRIO is not sold in North America. Please contact us to check the availability of this product in your country.

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