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Analysis of Fatty Acid Methyl Esters by GC

Data Subcutaneous Tissue Mass Imaging of
An epidemiological survey in the 1970s revealed that the indigenous Inuit
population in Greenland, which has a primary diet of seals and other marine
After derivatizing fatty acids into fatty acid methyl esters (FAMEs), the GC Analytical Conditions (General) animals, had a much lower death rate from cardiovascular disease than Danish
components are separated and detected by gas chromatography. A variety of Model : GC-2010 people. Analysis of
liquid phase columns with different polarities are available for GC analysis of Inj.Temp. : 250°C Furthermore, it was found that the Inuit blood contained high levels of Comprehensive
fatty acids, which can be selected for use based on the target components Carrier Gas : He 25 cm/sec eicosapentaenoic acid (EPA), large amounts which are contained in the fat of Glycerophospholipids
(Constant Linear Velocity Mode)
being separated. Figs. 1 and 2 show examples of using FAMEWAX (chemically Split Ratio : 1 : 25 fish and seals. This sparked an interest in fatty acids. The epidemiological
bonded polyethylene glycol) and BPX70 (70% cyanopropyl survey indicated that consuming large amounts of EPA might prevent
polysilphenylene-siloxane) columns and an FID detector to separate and detect cardiovascular diseases. *
components in a standard mixture containing 37 FAMEs. In addition to inhibiting the production of various eicosanoids from arachidonic
acid, Omega-3 fatty acids are also converted to a type of eicosanoid that is less
likely to cause blood platelet aggregation or inflammation than eicosanoids Technology
C6:0 C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1n9t+C18:1n9c from arachidonic acid. Consequently, omega-3 fatty acids are presumed to be Supercritical Fluid Lipid Analysis Using
effective at reducing blood platelet aggregation and inflammation caused by
eicosanoids derived from arachidonic acid.
C11:0 C13:0 C14:1 C15:0 C15:1 C16:1 C17:0 C17:1 C18:2n6t C18:2n6c C18:3n6 C18:3n3 C20:0 C20:1n9 C20:2n6 C20:3n6 C21:0 C20:4n6 C20:3n3 C22:0 C22:1n9 C22:2n6 C23:0 C24:0 C22:6n3 * Lancet. 1978 Jul 15;2(8081):117–9 Analysis of

C20:5n3
C24:1

Advanced Flow Technology Glycerophospholipids
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 35.0 min
Shimadzu’s proprietary Advanced Flow Technology products offer the ultimate in advanced gas flow control and flow switching
Column : FAMEWAX 30 m × 0.25 mm I.D. df : 0.25 µm Column Temp : 130 ˚C (0 min) – -230 ˚C at 5 ˚C/min
technology. In addition to further shortening analysis times and improving productivity, a family of high-sensitivity detectors is
Fig. 1 Example of Using a FAMEWAX Column to Analyze a 37-FAME Standard Mixture available to assist in reliably separating and quantitating even ultra-trace substances in a wide range of fields. Blood Serum Analysis of Lipid

GC-2010 Plus systems not only offer high reproducibility, they also feature a line of FPD, FID, and other detectors with sensitivity Mediators in Human
levels that are among the highest in the world to enable analyzing trace quantities with high reliability and high precision.
C6:0 C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1n9c C20:0 C20:4n6 C22:0 C24:0 These gas chromatograph systems also offer high-speed oven cooling, backflushing, and other features that can dramatically reduce
analysis times and improve analytical productivity.

C11:0 C13:0 C14:1 C15:0 C15:1 C16:1 C17:0 C17:1 C18:1n9t C18:2n6t C18:2n6c C18:3n6 C18:3n3 C20:1n9 C21:0 C20:2n6 C20:3n6 C20:3n3 C22:1n9 C20:5n3 C23:0 C22:2n6 C24:1 C22:6n3 of Glycolipids Structural Analysis

5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 35.0 37.5 min
Column : BPX70 60 m × 0.22 mm I.D. df : 0.25 µm Column Temp : 120 ˚C (0 min) – -250 ˚C at 3 ˚C/min Content of Human ES Cells Composition in Overall Lipid Analysis of Fatty Acid
Fig. 2 Example of Using a BPX70 Column to Analyze a 37-FAME Standard Mixture

Capillary Gas Chromatograph
GC-2010 Plus Esters by GC Acid Methyl Analysis of Fatty

Reference: Application News No. G252 “Analysis of 37 FAMEs using 6 Types of Capillary Columns” (LAAN-A-G-E013) Reference: GC-2010 Plus brochure (C184-E019)

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