Page 17 - LifeScience Solution for Lipid and Lipidome

Analysis of Fatty Acid Composition in Overall Lipid Content

of Human ES Cells Subcutaneous Tissue Mass Imaging of

Data
Docosahexaenoic acid (DHA) is a fatty acid present in large quantities in the
brain. It accounts for 40% of the fatty acids in the brain and 60% of the fatty
In an effort to establish practical applications for regenerative medicine, research is actively being conducted on ES cells and iPS cells. acids in the retina. Based on experiments with DHA-deficient mice, DHA
Due to the undeveloped mitochondria in undifferentiated ES cells, the primary energy source is from the glycolytic system. When deficiency is reported to affect brain and nerve system development. Analysis of
mitochondria develop as the ES cells become differentiated, the TCA cycle is said to become the energy source. Consequently, DHA has attracted attention as a component in dietary Comprehensive
This example describes the results from analyzing the composition of fatty acids in human ES cells. After extracting all cellular lipids supplements, which are claimed to have benefits such as reducing the risk of Glycerophospholipids
from frozen pellets of human ES cells, the fatty acids were methyl-esterified. Analyzing the methylated fatty acids by GC-MS resulted cardiovascular disease, reducing neutral fats in the blood, improving blood
in identification of 19 types of fatty acids. pressure, aiding growth and behavioral and visual development in infants, and
reducing the symptoms and frequency of depression. Many research results
(×10,000,000) have been reported on the effectiveness of such functions.
3.0
TIC Technology
2.5 Supercritical Fluid Lipid Analysis Using

2.0

1.5

1.0 Analysis of
UFMS Technology Glycerophospholipids
0.5 Gas chromatograph mass spectrometers (GC-MS) are especially
effective at analyzing the many different molecular species of Smart Metabolites Database
fatty acids. GC-MS analysis requires optimizing analytical
0.0 Pyruvic acid Acety
Acety
a
a
aci
ac
aci
a
Acetyl
aci
Acetyl C-CoA
45.0 50.0 55.0 60.0 65.0 conditions, such as the method used to derivatize analytical Citric acid
samples and the analytical column used. To eliminate tedious
aci
Fig. 1 TIC of Methyl Ester Derivatives from Human ES Cell Extract Sample Oxalacetic acid Aconitic acid d Blood Serum
jobs and streamline these analytical processes, Shimadzu has Mediators in Human Analysis of Lipid
a
acid
ac
ac
acid
cid
cid
c
cid
Malic
Malic acid
Malic
Malic
ali
l
acid
ali
ali
established a database of various fatty acid and metabolite Malic ic ic c c c acid i d d Isocitric acidd
Table 1 List of Detected Fatty Acid Methyl Esters components and offers columns and analytical conditions Fuumaricc acid d
m
uma
umari
m
m
c acid
m
F
Fuma
um
-Ket
2-Ketooglutarric
2-Ket o glut
suited to target compounds. accid
Compound Name Retention Time (min) Retention Index Similarity* S/N Using the Smart Metabolites Database allows GC-MS and Succinccinic acic acid d d Succinyl-CoA A
ucc
ucc
Succin
c
ic ac
cc
Succin
yl
C
Methyl myristate 41.925 2204 98 380 GC-MS/MS analysis of a wide variety of fatty acids.
Methyl pentadecanoate 43.755 2309 99 372
Methyl palmitate 45.505 2413 98 12739 of Glycolipids
Methyl palmitoleate 46.760 2490 95 143 Structural Analysis
Methyl margarate 47.150 2515 98 606
Methyl stearate 48.735 2619 98 29218
Methyl oleate 49.790 2689 96 96
Methyl linoleate 51.300 2793 99 1101
Methyl arachisate 51.690 2821 98 811
Methyl cis -11-icosenoate 52.675 2891 99 1602
Methyl linolenate 53.005 2913 98 122
Methyl cis -11,14-Icosadienoate 54.165 2991 98 124
Methyl eicosa-8,11,14-trienoate 55.315 3069 99 632 Analysis of Fatty Acid
Methyl erucate 55.535 3083 98 93 Content of Human ES Cells Composition in Overall Lipid
Methyl cis -11,14,14-Icosatrienoate 55.910 3108 97 24
Methyl arachidonate 56.240 3130 99 457
Methyl cis -5,8,11,14,17-Eicosapentaenoate 58.230 3254 99 243
Methyl cis -7,10,13,16,19-docosapentaenoate 61.995 3478 98 617
Methyl cis -4,7,10,13,16,19-Docosahexaenoate 63.150 3547 99 62
Triple Quadrupole Gas Chromatograph Mass Spectrometer
Methyl margarate-d3 47.085 2511 97 19864 GCMS-TQ8040
*Similarity reflects the results obtained in the NIST mass spectrum library search. Esters by GC Acid Methyl Analysis of Fatty
Reference: Technical Report “Determination of Fatty-acid Composition of Lipids in Human Embryonic Stem Cells Using GC-MS” (C146-E243) Reference: GCMS-TQ8040 brochure (C146-E251), Smart Metabolites Database flyer (C146-E277)
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