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Gas Chromatograph Triple Quadrupole

            Mass Spectrometer (GC/MS/MS)                                                                                              High Performance Liquid Chromatograph (HPLC)






                                                                                                                                      Triglycerides

                                                                                                                                      The Evaporative Light-Scattering Detector (ELSD-LT) is a universal detector for HPLC. The ELSD-LT can remove the mobile phase through
                                                                                                                                      evaporation and then make a light scattering measurement of the dried analyte particle.

                                                                                                                                      In principle, the ELSD-LT can detect all sample compounds that are not volatile ones, so it is ideally suited for those that have low
                                                                                                                                      absorbance and are difficult to detect using a UV detector such as sugars, fats and surfactants. Furthermore, it can be used for a
                                                                                                                                      wide range of applications, since it can be used with gradient elution for optimized separations and reduced run times, which is not
                                                                                                                                      possible with a Refractive Index Detector (RID). This example introduces an analysis triglycerides (triacylglycerols) contained in
            Representative MRM chromatogram where 3-MCPD-d5, 3-MCPD, 2-MCPD, glycidol-d5 and glycidol were eluted at 18.4 min, 18.6 min, 19.6 min, 21.6 min and 21.7 min, respectively.  cooking oil using the ELSD-LT.
            Reference: Determination of Fatty Acid Esters of 2- and 3-Monochloro-1,2-propanediol (MCPD) and Glycidol in Edible Oil Using GC/Triple Quadrupole MS
            Polycyclic Aromatic Hydrocarbons (PAHs)                                                                                   Analytical Conditions

            In recent years, there has been a trend toward viewing high concentrations of polycyclic aromatic hydrocarbons (PAHs) in foods   Column   :Shim-pack VP-ODS (250 mmL. × 4.6 mmI.D.)
            (particularly smoked products) as a problem. In 2009, the Codex Alimentarius Commission issued standards for reducing the PAH   Mobile phase   :Acetonitrile/ Acetone=3 / 7(v/v)
                                                                                                                                      Flow rate
                                                                                                                                                    :1.0 mL/min
            contamination level in smoked and directly dried foods. In addition, Europe, Canada, South Korea, China, and others established food   Column temperature  :30°C
            standards for benzo[a]pyrene. Shown here is an example of GC-MS/MS analysis of PAHs in "katsuobushi" (dried, smoked, and   Detection    :ELSD-LT
            fermented skipjack tuna, which is very popular in Japanese cuisine).                                                      Temperature    :35°C
                                                                                                                                      GAIN          :5
            Analytical Conditions                                                (Left) GC-MS (SIM)  (Right) GC-MS/MS (MRM)           Nebulizer gas    :Air
                                                                        Benzo[a]                                                      Gas pressure    :350 KPa
            GC-MS           :GCMS-TQ8040                                 pyrene
            Column          :Rxi-PAH (60 m long, 0.25 mm I.D., df = 0.1 µm) (RESTEK, P/N: 49317)
            Glass insert    :Splitless insert with wool (Shimadzu, P/N: 221-48876-03)
            [GC]                                   [MS]
            Injection volume   :2 µL               Interface temperature :330ºC
            Injection unit temperature :330ºC      Ion source temperature :230ºC  Indeno
            Column oven temperature :180ºC (2 min)     (5ºC /min)       Measurement mode  :MRM  [1,2,3-cd]
                             260ºC     (15ºC/min)     350ºC (12 min) Loop time   :0.3 sec  pyrene
            Carrier gas control   :Constant linear velocity (40.0 cm/sec)
            Injection mode   :Splitless
            Sampling time   :2 min
            Carrier gas     :Helium                                     SIM and MRM Chromatograms of PAHs in Katsuobushi Extract

            Reference: Application News MS109 - Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) in Foods by GC-MS/MS
            Shimadzu Gas Chromatograph Triple Quadrupole Mass Spectrometer Product Lineup


                      Model                                            Features
                                      · GCMS-TQ8040 with MRM Optimization Tool seamlessly selects and optimizes MRM transitions for multiple
                                        compounds in a single sequence
                                      · Smart Database series provides fully automated method development at the touch of a button
                                      · Smart MRM method creation protocol automatically sets the optimum dwell and loop times for each method to maximize sensitivity
                                      · Flexible MS Events prevent losing compounds in the presence of a complex matrix
                                      · Scan/MRM analysis mode simultaneously acquires accurate library-searchable mass spectra and low-level MRM quantitation in a single analysis
                GCMS-TQ8040           · Automatic Adjustment of Retention Times (AART) with > 99.9% accuracy means no need to adjust flow rates or update RTs manually


                                      · Better amplification performance that maximizes use of Off-Axis Ion Optics made possible by new long-life detector
                                      · Contamination-resistant ion source coupled with a new long life-detector ensures reliable analysis with less maintenance requirements
                                      · Large-capacity turbomolecular pump with improved exhaust efficiency allows for higher sensitivity and stability                Chromatogram of a Standard Mixture of Triglycerides
                                      · Smart MRM technologies coupled with the use of LabSolutions Insight improve ease of method creation and data review                    (upper: ELSD-LT, lower: RID)
                                      · High-speed scan and data acquisition using Advance Scanning Speed Protocol (AASP) to achieve speeds up to 20,000 µ/sec across all masses
                                      · Ecology mode reduces costs associated with power and carrier gas consumption
                GCMS-TQ8050           · Various configurations enable optimal performance for specific analytical needs
                                                                                                                                      Reference: Application News No. L290A  Application of Evaporative Light-Scattering Detector “ELSD-LT” (Part1) Analysis of Triglycerides in Cooking Oil

                                                                                                                                                                   Solutions for Palm Oil Analysis Application Notebook
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