Page 20 - Food&Beverages Solution Palm Oil

Gas Chromatograph Triple Quadrupole

Gas Chromatograph Mass Spectrometer (GCMS) Mass Spectrometer (GC/MS/MS)

MCPD MCPD

3-MCPD (3-Chloropropane-1,2-diol) is a carcinogenic toxic compound and suspected to be genotoxic to humans. It is found in various Esterified and free forms of 2-MCPD, 3-MCPD and glycidol are heat-induced contaminants found in various types of processed food.
foods, such as acid hydrolyzed vegetable protein (HVP), soy sauce and products made of refined vegetable oils. The esters of 3-MCPD are Following ingestion, esterified forms of these compounds are metabolized to their respective free forms, which have been associated
also formed at high temperature during the refining process of edible oils and fats and are readily metabolized to 3-MCPD after with a range of toxicities such as carcinogenicity and nephrotoxicity.
consumption. In 2006 the European Commission established a tolerable daily intake (TDI) of 2ug/kg for 3-MCPD and derivatives, and
making it necessary to monitor and report the level of 3-MCPD in certain foods. Various studies have investigated the levels of these contaminants in oils/fats from different sources and mitigation strategies are
currently under development. Well-validated analytical methods are critical for these studies and currently, the American Oil Chemists’
Analytical Conditions Society (AOCS) has adopted three methods for the analysis of these contaminants in edible oils and fats using gas
[GC] [MS] chromatography-mass spectrometry (GCMS). To support future studies where higher sensitivity and selectivity are necessary, analytical
Injection volume :1.0 µL Ion source temperature :200°C solutions based on triple-quadrupole mass spectrometry will be required. In the present study, a novel gas chromatography-triple
Injection mode :Splitless Interface temperature :300°C quadrupole mass spectrometry (GC/TQMS) method was developed and validated for the simultaneous analysis of 2-MCPD, 3-MCPD and
Injection temperature :270°C Measurement mode :SIM glycidyl fatty acid esters in edible oil. Subsequently, this method was applied for the quantitation of these contaminants in commercial
Column oven temperature :85°C - (6°C/min) - 150°C - (12°C/min) - 180°C - (25°C/min) - 280°C [Compounds Monitoring lons] edible oil samples.
He gas flow :1 ml/min 3-MCPD-D5 m/z 150*,201
Modulation time :8 sec 3-MCPD m/z 147*,196
Hot pulse time :0.5 sec (325°C) *Quantitative lons

Analytical Conditions
[GC] [MS]
Injection volume :0.5 µL Ion source temperature :230°C
Injection mode :Splitless Interface temperature :300°C
Injection temperature :250°C Measurement mode :MRM
Column oven temperature :80°C(1 min) - (10°C/min) - 170°C(5 min) - (3°C/min) - 200°C -
(15°C/min) - 300°C(15 min)
Control mode :Constant pressure (49.7 kPa)

3-MCPD spiked at 2 µg/g in palm oil; 3-MCPD-D5 was added as an internal standard and analyzed with the GCMS-QP2020 MRM Parameters

Reference: Flyers (SAP-GCMS-QP2020-3-MCPD-01) – 3-MCPD in edible oils and fats

Shimadzu Gas Chromatograph Mass Spectrometer Product Lineup

Model Features

· High-performance ion source and ion optics enhance ionization and transport efficiency
· Large-capacity turbomolecular pump with improved exhaust efficiency that improves not only use with
helium but also hydrogen and nitrogen
· Easily switch between EI and PCI methods using new Quick-CI feature
TM
· High-speed scan and data acquisition using Advance Scanning Speed Protocol (AASP ) to achieve
speeds up to 20,000 µ/second across all masses
· Ecology mode reduces costs associated with power and carrier gas consumption
GCMS-QP2020 · Ability for various configurations for optimal analytical needs

· Scanning speeds up to 10,000 µ/second, and includes three operation modes: Scan, SIM, and Scan/SIM.
· The ion source is equipped with an automatic-switching dual filament to minimize maintenance.
· Routine analyses are fully automated using a range of sample introduction techniques, including
large-volume and temperature-programmable injections, direct insertion probe, headspace, purge-and-trap,
and others
· Robust single turbomolecular pump system that allows for twin-line configurations, helping your lab to
GCMS-QP2010 SE maximize efficiency
· Ecology mode reduces costs associated with power and carrier gas consumption

Solutions for Palm Oil Analysis Application Notebook
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