Page 23 - Shimadzu Journal vol.2 Issue1

Poster 11 ASMS
Strategies for structure elucidation using Ultrafast Mass Spectrometry (UFMS):
Using nMS as an alternative to MS 3
2
We have applied UFMS to generate multiple product ion spectra (PIS) across a UHPLC peak with CID at varying energies. The nMS2
approach yields an array of MSMS spectra derived from the same parent ion but exhibiting mechanistically distinct fragment ions.
UFMS has enabled on-the-fly detection and identification of novel compounds (e.g. metabolites) with arrayed MS2 data.
Unlike MSn, the nMS2 approach is not subject to the transfer loss limitations on sensitivity because the same parent ion is selected
from the first mass filter rather than from collisional debris.

Poster 12 ASMS
Novel bacterial classification method by MALDI-TOF MS based on ribosomal protein
coding in S10-spc-alpha Operon at Strain level
We developed a novel bacterial classification method by MALDI-TOF MS using ribosomal subunit proteins coded in
S10-spc-alpha operon as biomarkers, because the operon encodes half of the ribosomal subunit and can be easily sequenced
using conventional DNA sequencer. Now we are trying to differentiate other bacterial species that are difficult to discriminate
using the mass fingerprinting method. These bacterial species under our evaluation are important in the area of food safety
so we expect that our trial will contribute to this area.

Poster 13 HPLC

Novel Methods for Detection and Quantitation of Impurities Using a New High Sensitivity
Photodiode Array Detector
The presence of impurities in commercial products may seriously affect their quality, resulting in more stringent requirements
for impurity monitoring. For this reason, researchers require a more sensitive UHPLC detector. We have developed a new
high-sensitivity photodiode array detector with novel data processing methods to detect and quantitate very trace amounts of
impurities present in complex samples.

Poster 14 HPLC
Screening of 25-OH Vitamin D2,D3 using Shimadzu LCMS-8040
Hypovitaminosis D is a relatively common problem traditionally manifesting in the elderly population and in people with
severe liver or kidney disease. Inadequate levels of vitamin D can lead to increased risk of cancer, diabetes mellitus, chronic
pain, and hypertension. We evaluated Shimadzu's Triple Quadrupole LCMS-8040 using different commercial kits available for
detection of 25-OH Vitamin D and a home-brew method using a considerable number of biological samples.

Poster 15 HPLC
Glycerophospholipids Analysis by Two-Dimensional Liquid Chromatography Coupled
with a Triple Quadrupole Mass Spectrometer
Glycerophospholipids (GPLs) are the major component of biological membranes. They can not only act as a barrier from the
external environment, but can also play a key role in a variety of biological processes including membrane trafficking and
signal transduction. Thus, analysis of GPLs is one of the most important studies in the metabolomics field. We established a
new strategy using two-dimensional liquid chromatography (2D-LC) coupled with ESI-MS/MS (2D-LC-ESIMS).

49