Page 28 - Shimadzu Journal vol.2 Issue1

Topics

At Eisai, Yamamoto et al. analyzed a liposome preparation with an 8. Conclusion
SPE-SPE-HPLC system utilizing MAYI-ODS. In this research, it was Using a system combining the Shim-pack MAYI biological sample
possible to simultaneously quantify liposomal doxorubicin and pretreatment column and Co-Sense for BA, plasma, serum and
released doxorubicin in plasma, and this has even been applied to other biological samples can be injected directly in large volumes
a PK study utilizing mice [15].
for analysis. High-concentration proteins and electrolytes,
unnecessary components, are almost completely removed, and
stable data can be obtained even for unstable components, with
7. Ideal in Combination with Shimadzu UFMS Series LC/MS
no loss of precious samples. In addition, the adoption of
Speed and Sensitivity Beyond Comparison Shimadzu's proprietary dilution trap method maintains peak shape
The LCMS-8050 triple quadrupole mass spectrometer is the top and enhances sensitivity by concentrating the intended
model in the Shimadzu UFMS series. It combines the world's components, thereby providing stable recovery rates and
fastest speeds with the highest class of sensitivity. It provides 5 reproducibility. Furthermore, the system demonstrates its
msec ultra-fast positive/negative ionization switching and 30,000 effectiveness in protecting analysis columns and the MS interface,
u/sec ultra-fast scanning performance. As a result, quantitative and reducing matrix effects. In terms of items required for method
and qualitative measurements can be performed in parallel in a validation, it contributes to the efficient attainment of highly
single analysis by combining MRM and scan measurements. reliable results. In the future, its application will likely extend to
Quantitative accuracy is not lost even in such simultaneous other types of analysis, including impurities related to ADCs and
MRM/scan measurements. other biopharmaceuticals.
Accordingly, highly reliable quantitative results and reliable
qualitative information can always be obtained. In addition to high References
quantitative throughput, the system also demonstrates its
strengths in the process of screening and optimizing analysis [1] N.M. Cassiano, J.C. Barreiro, M.C. Moraes, R.V. Oliveira, Q.B.
conditions. Cass, Bioanalysis 1 (2009) 577.
Furthermore, a contamination-resistant ionization interface has [2] S.H. Yang, H. Fan, R.J. Classon, K.A. Schug, J. Sep. Sci. 36
been adopted, and the design focuses on the robustness of the (2013) 2922.
system as a whole. Fig. 12 is a plot of area values, in which plasma [3] N. Asakawa, H. Ohe, M. Tsuno, Y. Nezu, Y. Yoshida, and T.
samples spiked with alprazolam were deproteinized, and then Sato, J. Chromatogr., 1991, 541, 231
analyzed consecutively 1,000 times. From the 1,000 data cycles, [4] Y. Oda, N. Asakawa, T. Kajima, Y. Yoshida, and T. Sato, J.
an excellent area value reproducibility of %RSD 4.59 % was Chromatogr., 1991, 541,411
obtained. [5] E. Yamamoto, K. Murata, Y. Ishihama, N. Asakawa, Analytical
Sciences (2001), 17(10), 1155-1159.
[6] S. Kawano, H. Murakita, E. Yamamoto, N. Asakawa, J. Chro-
matogr B (2003), 792(1), 49-54.
[7] E. Yamamoto, T. Sakaguchi, T. Kajima, N. Mano, N. Asakawa,
J. Chromatogr B (2004), 807(2), 327-334.
[8] S. Kawano, M. Takahashi, T. Hine, E. Yamamoto, N. Asakawa,
Rapid Communications in Mass Spectrometry (2005), 19(19),
2827-2832.
[9] T. Sakaguchi, E. Yamamoto, I. Kushida, T. Kajima, N. Asaka-
Fig. 12 1,000 Consecutive Analyses of a Deproteinized Blood
Plasma Sample Spiked with Alprazolam (LCMS-8050) wa, J. Pharm. Biomed. Anal. (2006), 40(2), 345-352.
[10] E. Yamamoto, S. Takakuwa, T. Kato, N. Asakawa, J. Chro-
matogr. B (2007), 846(1-2), 132-138.
In analyses involving plasma and other biological samples, ease of
maintenance is an important point. With the LCMS-8050, the ESI [11] Y. Sato, E. Yamamoto, S. Takakuwa, T. Kato, N. Asakawa, J.
capillary is easily replaced, and the desolvation line can also be Chromatogr. A (2008), 1190(1-2), 8-13.
replaced without compromising vacuum, keeping downtime to a [12] E. Yamamoto, H. Igarashi, Y. Sato, I. Kushida, T. Kato, T.
minimum. In addition, Co-Sense for BA does not require special Kajima, N. Asakawa, J. of Pharm. Biomed. Anal. (2006), 42(5),
software, so with LabSolutions LCMS, everything from front-end 587-592.
LC to LCMS can be controlled centrally from a single PC. [13] E. Yamamoto, T. Kato, N. Mano, N. Asakawa, J. of Pharm.
Biomed. Anal. (2009), 49(5), 1250-1255.
[14] www.fda.gov/downloads/AboutFDA/.../UCM341177.pdf
[15] E. Yamamoto, K. Hyodo, N. Ohnishi, T. Suzuki, H. Ishihara, H.
Kikuchi, N. Asakawa, J. of Chromatogr. B (2011), 879(30),
3620-3625.

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